Difference between revisions of "Part:BBa M50436:Experience"
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===Applications of BBa_M50436=== | ===Applications of BBa_M50436=== | ||
| − | This protein can be used to speed up enterobactin synthesis slightly. | + | This protein can be used to speed up enterobactin synthesis slightly in ''E. coli''. It works in tandem with entC (BBa_M50435), facilitating the transformation of chorismate to isochorismate. |
===User Reviews=== | ===User Reviews=== | ||
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| − | We used this sequence as a part of a 2,3-DHB Biosynthesis construct (Part:BBa_M50437). We ordered the plasmid from DNA2.0 and transformed it into ''E. coli'', then plated the ''E. coli'' on LB + kanamycin. We saw colonies successfully grow up. We also ran a Western Blot for this protein and a saw a band of the expected size, indicating this protein was successfully synthesized. | + | We used this sequence as a part of a 2,3-DHB Biosynthesis construct (Part:BBa_M50437). We ordered the plasmid from DNA2.0 and transformed it into ''E. coli'', then plated the ''E. coli'' on LB + kanamycin. We saw colonies successfully grow up. We also ran a Western Blot for this protein and a saw a band of the expected size, indicating this protein was successfully synthesized. See https://parts.igem.org/Part:BBa_M50437:Experience for a detailed assessment. |
Latest revision as of 01:01, 12 June 2018
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Applications of BBa_M50436
This protein can be used to speed up enterobactin synthesis slightly in E. coli. It works in tandem with entC (BBa_M50435), facilitating the transformation of chorismate to isochorismate.
User Reviews
UNIQ94c22debb55681a9-partinfo-00000000-QINU UNIQ94c22debb55681a9-partinfo-00000001-QINU
We used this sequence as a part of a 2,3-DHB Biosynthesis construct (Part:BBa_M50437). We ordered the plasmid from DNA2.0 and transformed it into E. coli, then plated the E. coli on LB + kanamycin. We saw colonies successfully grow up. We also ran a Western Blot for this protein and a saw a band of the expected size, indicating this protein was successfully synthesized. See https://parts.igem.org/Part:BBa_M50437:Experience for a detailed assessment.