Difference between revisions of "Part:BBa M50096"
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<partinfo>BBa_M50096 short</partinfo> | <partinfo>BBa_M50096 short</partinfo> | ||
| − | EL-222 | + | EL-222 is a light-sensitive transcription factor that binds to DNA and drives transcription under blue light illumination. EL-222 consists of a LOV-HTH (helix-turn-helix) domain and VP16 activating unit. The LOV-HTH allows for light-gating of DNA binding; the LOV portion of the protein covers the HTH portion of the protein that binds DNA - under blue light exposure, the LOV domain changes conformation and reveals the HTH DNA binding site. The HTH binds to a sequence of c120 repeats. Once bound, the VP16 activating unit promotes transcription factors in the cell to drive genes downstream of the c120 repeats. |
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Latest revision as of 01:24, 14 December 2017
EL-222 - Light-sensitive transcription factor
EL-222 is a light-sensitive transcription factor that binds to DNA and drives transcription under blue light illumination. EL-222 consists of a LOV-HTH (helix-turn-helix) domain and VP16 activating unit. The LOV-HTH allows for light-gating of DNA binding; the LOV portion of the protein covers the HTH portion of the protein that binds DNA - under blue light exposure, the LOV domain changes conformation and reveals the HTH DNA binding site. The HTH binds to a sequence of c120 repeats. Once bound, the VP16 activating unit promotes transcription factors in the cell to drive genes downstream of the c120 repeats.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 463
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 33
- 1000COMPATIBLE WITH RFC[1000]