Difference between revisions of "Part:BBa K2379003:Design"
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=BioBrick Type= | =BioBrick Type= | ||
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===Source=== | ===Source=== | ||
Latest revision as of 03:54, 2 November 2017
RNA pHmeter with biobrick scar site
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 118
Design Notes
In order to retain its functionality, the secondary structure of the RNA pHmeter must be maintained which is destroyed by the replacement of the natural scar site with the biobrick one. Hence we have tried to alter the sequence such that its predicted secondary structure is conserved. The structures of the wild-type and modified RNA pHmeter as predicted by RNA VIENNA FOLD are shown below.
Wild Type
BioBrick Type
Source
[Escherichia coli UMN026 (strain: UMN026)] Genbank Gene ID: 7159109
References
1. Nechooshtan, G., Elgrably-Weiss, M., Sheaffer, A.,Westhof, E., & Altuvia, S. A pH-responsive riboregulator.Genes Dev. 23, 2650–2662 (2009).
2. Nechooshtan, G., Elgrably-Weiss, M., & Altuvia, S.Changes in transcriptional pausing modify the folding dynamics of the pH-responsive RNA element. Nucleic Acids research. 42(1), 622-630 (2013)
3. Bingham, R., Karen, H., Joan S. Alkaline induction of a Novel Gene Locus, alx, in Escherichia coli. Journal of Bacteriology. 172(4), 2184-2186(1990)