Difference between revisions of "Part:BBa K2309028:Experience"
 
(One intermediate revision by the same user not shown)
Line 2: Line 2:
 
__NOTOC__
 
__NOTOC__
  
−
<h2>1. Gel electrophoresis</h2>
+
We use gel electrophoresis to show the result of construction.
−
        </div>
+
  
−
        <div class="fig" align="center">
+
https://static.igem.org/mediawiki/2017/4/43/Gel_electrophoresis.jpeg">
−
            <figure>
+
 
−
                <img width="650px" src="https://static.igem.org/mediawiki/2017/4/43/Gel_electrophoresis.jpeg">
+
From left to right: pSB1C3+LL-37 (cut by PstI, uncut); pSB1C3+GF-17 (cut by pstI, uncut); 1kb DNA maker; pEt28a (+)+3x AMPs (uncut, cut)
−
                <figcaption>
+
−
                    <strong>Fig.1 From left to right: pSB1C3+LL-37 (cut by PstI, uncut); pSB1C3+GF-17 (cut by pstI, uncut); 1kb DNA maker;
+
−
                        pEt28a (+)+3x AMPs (uncut, cut)</strong>
+
  
  

Latest revision as of 03:35, 2 November 2017


We use gel electrophoresis to show the result of construction.

Gel_electrophoresis.jpeg">

From left to right: pSB1C3+LL-37 (cut by PstI, uncut); pSB1C3+GF-17 (cut by pstI, uncut); 1kb DNA maker; pEt28a (+)+3x AMPs (uncut, cut)


The inhibitio ring is used for test the production of peptide to S. aureus

3P.png

the results of MIC for mixed peptide as follows:

Test11.png

for the biofilm formation assay, the result was shown as follows:

Abs_123xjtlu.png

Applications of BBa_K2309028

User Reviews

UNIQc34cd5dea0fcaa18-partinfo-00000000-QINU UNIQc34cd5dea0fcaa18-partinfo-00000001-QINU