Difference between revisions of "Part:BBa K2378009"
 
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<partinfo>BBa_K2378009 short</partinfo>
 
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This is coding device of endonuclease degrades free extracellular DNA, with constitutive promoter BBa_J61051. The gene is isolated from <i>Vibrio cholera</i>.
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This is coding device of EndA DNA-specific endonuclease, with constitutive promoter BBa_J61051. The gene is isolated from <i>Vibrio cholera</i>.
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===Usage and Biology===
 
===Usage and Biology===
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<p>EndA endonuclease is expressed entirely extracellularly. Its natural function in nature is to prevent transformation when expressed, so that <i>Vibrio cholera</i> can manage the period and seasons for transformation.</p>
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<p>Utilizing that characteristics, this enzyme can be used to coat bacterial cells in endonuclease cloud. This can prevent natural transformation from happening. This function is particularly useful as a containment/safety device to prevent transformation of natural organisms by genetically modified ones which can disrupt the natural gene pool. </p>
  
 
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Latest revision as of 01:22, 2 November 2017


EndA Endonuclease Coding Device from Vibrio cholera

This is coding device of EndA DNA-specific endonuclease, with constitutive promoter BBa_J61051. The gene is isolated from Vibrio cholera.


Usage and Biology

EndA endonuclease is expressed entirely extracellularly. Its natural function in nature is to prevent transformation when expressed, so that Vibrio cholera can manage the period and seasons for transformation.

Utilizing that characteristics, this enzyme can be used to coat bacterial cells in endonuclease cloud. This can prevent natural transformation from happening. This function is particularly useful as a containment/safety device to prevent transformation of natural organisms by genetically modified ones which can disrupt the natural gene pool.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 305
    Illegal NgoMIV site found at 545
  • 1000
    COMPATIBLE WITH RFC[1000]