Difference between revisions of "Part:BBa K2355037"
 
(2 intermediate revisions by 2 users not shown)
Line 3: Line 3:
 
<partinfo>BBa_K2355037 short</partinfo>
 
<partinfo>BBa_K2355037 short</partinfo>
  
−
This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of Bitis gabonica. The specific peptide sequence of the linker is GGVRGPRV.  
+
This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of <i>Bitis gabonica</i>.  
 +
The specific peptide sequence of the linker is GGVRGPRV.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
Line 21: Line 22:
  
 
This peptide is cleaved only by <i>Bitis gabonica</i> and can be used as a single positive response for that snake.
 
This peptide is cleaved only by <i>Bitis gabonica</i> and can be used as a single positive response for that snake.
 +
 +
In the graf below you are able to see the cleavage by all three venoms.
 +
<html>
 +
 +
<figure>
 +
              <img src="https://static.igem.org/mediawiki/2017/0/03/T--DTU-Denmark--peptidescreenO22.png" alt="Substrate screening data for O22" width="500">
 +
              <figcaption>Figure 12: Substrate with sequence GGVRGPRV (well O22) incubated with the three different venoms.</figcaption>
 +
          </figure>
 +
 +
</html>

Latest revision as of 00:22, 2 November 2017


Cleavable peptide sequence from Human fibrinogen alpha chain

This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of Bitis gabonica. The specific peptide sequence of the linker is GGVRGPRV.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Results of experiments

This peptide is cleaved only by Bitis gabonica and can be used as a single positive response for that snake.

In the graf below you are able to see the cleavage by all three venoms.

Substrate screening data for O22
Figure 12: Substrate with sequence GGVRGPRV (well O22) incubated with the three different venoms.