Difference between revisions of "Part:BBa K2355014"
 
(One intermediate revision by one other user not shown)
Line 22: Line 22:
  
 
This peptide is cleaved by <i>Bitis arietans</i> and <i>Bitis gabonica</i>.
 
This peptide is cleaved by <i>Bitis arietans</i> and <i>Bitis gabonica</i>.
−
They can be used to distinguish between these two Bitis snakes and <i>Naja nigricollis</i>.
+
It can be used to distinguish between these two Bitis snakes and <i>Naja nigricollis</i>.
 +
 
 +
In the graf below you are able to see the cleavage by all three venoms.
 +
 
 +
<html>
 +
<figure>
 +
              <img src="https://static.igem.org/mediawiki/2017/9/9b/T--DTU-Denmark--peptidescreenD19.png" alt="Substrate screening data for D19" width="500" >
 +
              <figcaption>Figure 1: Substrate with sequences PEDDGYFV (well D19) incubated with the three different venoms.</figcaption>
 +
          </figure>
 +
</html>

Latest revision as of 00:17, 2 November 2017


Cleavable peptide sequence from Human DNA topoisomerase 1

This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of Bitis gabonica and Bitis arietans. The specific peptide sequence of the linker is PEDDGYFV.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Results of experiments

This peptide is cleaved by Bitis arietans and Bitis gabonica. It can be used to distinguish between these two Bitis snakes and Naja nigricollis.

In the graf below you are able to see the cleavage by all three venoms.

Substrate screening data for D19
Figure 1: Substrate with sequences PEDDGYFV (well D19) incubated with the three different venoms.