Difference between revisions of "Part:BBa K2381012"
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<partinfo>BBa_K2381012 short</partinfo>  
 
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It’s a gRNA sequence containing 20bp targeting at <i>oriC</i> and a full-length gRNA scaffold. The sequence is central to our project and is related with all the system built this year.<br/>
 
It’s a gRNA sequence containing 20bp targeting at <i>oriC</i> and a full-length gRNA scaffold. The sequence is central to our project and is related with all the system built this year.<br/>
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The targeting sequence of this part is acquired from xxxx. Its function after J23119 promoter, <partinfo>BBa_K2381020</partinfo>, is proved by OD measurement after cotransformed with plasmid containing dCas9.<img src ="https://static.igem.org/mediawiki/parts/d/d2/T--HZAU-China--gRNA_function.png"> We can observe tight repression on cell growth. Further investigation is conducted by qPCR and flow cytometric.<img src ="https://static.igem.org/mediawiki/parts/2/26/T--HZAU-China--gRNA_qPCR.png">
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The targeting sequence of this part is acquired from reference paper<sup>1</sup>.  
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Its function after light-induced promoter, <partinfo>BBa_K2381020</partinfo>, is proved by OD measurement. <img src ="https://static.igem.org/mediawiki/parts/f/f1/T--HZAU-China--light_gRNA.png">
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<img src="https://static.igem.org/mediawiki/parts/d/d2/T--HZAU-China--gRNA_function.png" width="750px"/>
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<b>BBa_K2381024</b>, is proved by OD measurement after cotransformed with plasmid containing dCas9. Its effect is even better than rapamycin, a well-used chemical molecule for replication inhibition.
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<img src="https://static.igem.org/mediawiki/parts/2/26/T--HZAU-China--gRNA_qPCR.png" width="750px"/>
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Further investigation is conducted by qPCR and flow cytometric.
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<img src="https://static.igem.org/mediawiki/parts/f/f1/T--HZAU-China--light_gRNA.png" width="750px"/>
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Its function after light-induced promoter, <b>BBa_K2381023</b>, is proved by OD measurement.
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<h3>References</h3>
 
<h3>References</h3>
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1. Wiktor, J., Lesterlin, C., Sherratt, D. J., & Dekker, C. (2016). CRISPR-mediated control of the bacterial initiation of replication. Nucleic Acids Res, 44(8), 3801-3810.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 01:00, 18 October 2018


3'-OriC gRNA

It’s a gRNA sequence containing 20bp targeting at oriC and a full-length gRNA scaffold. The sequence is central to our project and is related with all the system built this year.
The targeting sequence of this part is acquired from reference paper1.


BBa_K2381024, is proved by OD measurement after cotransformed with plasmid containing dCas9. Its effect is even better than rapamycin, a well-used chemical molecule for replication inhibition.



Further investigation is conducted by qPCR and flow cytometric.



Its function after light-induced promoter, BBa_K2381023, is proved by OD measurement.


References

1. Wiktor, J., Lesterlin, C., Sherratt, D. J., & Dekker, C. (2016). CRISPR-mediated control of the bacterial initiation of replication. Nucleic Acids Res, 44(8), 3801-3810.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]