Difference between revisions of "Part:BBa K2398009"
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− | This part provide bacteriophage M13 | + | This part provide bacteriophage M13 geneVI with a relatively weak RBS, sd8[[#References|[1]]], flanked by two homology regions for the usage due to the cloning standard of the iGEM Team Heidelberg 2017 (http://2017.igem.org/Team:Heidelberg/RFC). Figure one gives a short overview of our standard. Our BioBricks from the registry can easily be used for the assembly of blasmid with the standard (Fig.: 2). |
[[File:T--Heidelberg--Team_Heidelberg_2017_RFC_hd-1.jpeg|thumb|center|Figure 1: In our cloning standard, compatible building blocks are defined by specific functionalities. They are flanked by defined homology regions, indicated by numbers, which are necessary for the assembly of the APs with the Gibson method. This results in a highly customizable plasmid, composed of the desired origin of replication, an antibiotic resistance (4-5), a bicistronic operon with geneIII (2-3)and the desired reporter (3-4), which can be activated by any promoter (1-2)and a second expression cassette for additional genes that are necessary for the respective circuit (1-5). ]] | [[File:T--Heidelberg--Team_Heidelberg_2017_RFC_hd-1.jpeg|thumb|center|Figure 1: In our cloning standard, compatible building blocks are defined by specific functionalities. They are flanked by defined homology regions, indicated by numbers, which are necessary for the assembly of the APs with the Gibson method. This results in a highly customizable plasmid, composed of the desired origin of replication, an antibiotic resistance (4-5), a bicistronic operon with geneIII (2-3)and the desired reporter (3-4), which can be activated by any promoter (1-2)and a second expression cassette for additional genes that are necessary for the respective circuit (1-5). ]] |
Latest revision as of 02:14, 2 November 2017
sd8-geneVI from M13 bacteriophage
This part provide bacteriophage M13 geneVI with a relatively weak RBS, sd8[1], flanked by two homology regions for the usage due to the cloning standard of the iGEM Team Heidelberg 2017 (http://2017.igem.org/Team:Heidelberg/RFC). Figure one gives a short overview of our standard. Our BioBricks from the registry can easily be used for the assembly of blasmid with the standard (Fig.: 2).
![](/wiki/images/c/cf/T--Heidelberg--Team_Heidelberg_2017_RFC_hd-1.jpeg)
![](/wiki/images/c/c4/T--Heidelberg--Team_Heidelberg_2017_RFC_hd.jpeg)
This part was used in the context of a subproject of the iGEM Team Heidelberg 2017 (http://2017.igem.org/Team:Heidelberg/CRISPR).
Figure 3 gives a short overview on how the plasmid was used.
![](/wiki/images/3/30/T--Heidelberg--Team_Heidelberg_2017_pampace1.png)
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1
Illegal BglII site found at 455 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Rferences
[1] Ringquist, S.; Shinedling, S.; Barrick, D.; Green, L.; Binkley, J.; Stormo, G. D.; Gold, L. (1992): Translation initiation in Escherichia coli: sequences within the ribosome-binding site. In: Molecular microbiology 6 (9), S. 1219–1229.