Difference between revisions of "Part:BBa K2271107:Experience"
 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
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how you used this part and how it worked out.
 
  
 
===Applications of BBa_K2271107===
 
===Applications of BBa_K2271107===
  
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[[Image:Artico r19lvl2col.png|thumb|center|'''Figure 1:'''PEX5 knock out strain, transformed with the R19 variant, mTurqouise tagged with the PTS1 and the PEX13 membrane anchor fused to mRuby.]]
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[[Image:Artico r19lvl2col.png|thumb|center|'''Figure 1:'''PEX5 knock out strain, transformed with the R19 variant, mTurqouise tagged with the PTS1* P* and the PEX13 membrane anchor fused to mRuby.]]
 
[[Image:Artico wtpwtpstar.png|thumb|center|'''Figure 2:'''PEX5 knock out strain, transformed with the R19 variant, mTurqouise tagged with the PTS1 and the PEX13 membrane anchor fused to mRuby.]]
 
[[Image:Artico wtpwtpstar.png|thumb|center|'''Figure 2:'''PEX5 knock out strain, transformed with the R19 variant, mTurqouise tagged with the PTS1 and the PEX13 membrane anchor fused to mRuby.]]
  

Latest revision as of 21:15, 1 November 2017


Applications of BBa_K2271107

Figure 1:PEX5 knock out strain, transformed with the R19 variant, mTurqouise tagged with the PTS1* P* and the PEX13 membrane anchor fused to mRuby.
Figure 2:PEX5 knock out strain, transformed with the R19 variant, mTurqouise tagged with the PTS1 and the PEX13 membrane anchor fused to mRuby.

The figures 1 and 2 show the functionality of our Pex5 variant R19. R19 with PTS1* leads to clear import whereas the controls with the wild type PTS1 and the wild type Pex5 with PTS1* do not show any signs for import into the peroxisome.

User Reviews

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