Difference between revisions of "Part:BBa K2384015:Design"
 
Line 8: Line 8:
 
===Design Notes===
 
===Design Notes===
  
−
CDS sequence optimization in BBa_K538004 is better suited for expression in Bacillus megaterium
+
Tightly regulated promoter and efficiently inducible expression
  
  
 
===Source===
 
===Source===
−
This gene is a cold resistant gene from the Arctic cryophile Polaribacter irgensii from BBa_K538004 .
+
BBa_K733002 BBa_K346004 BBa_K823037
  
 
===References===
 
===References===

Latest revision as of 17:55, 1 November 2017


Repressor gene xylR+Strong PxylA promoter+RBS+Lpp-Omp-MBP(Pb)+His-tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 847
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Tightly regulated promoter and efficiently inducible expression


Source

BBa_K733002 BBa_K346004 BBa_K823037

References