Difference between revisions of "Part:BBa K2384008"
 
(2 intermediate revisions by the same user not shown)
Line 7: Line 7:
  
 
===Usage and Biology===
 
===Usage and Biology===
−
<br/><br/>
+
 
 
Asparagine chemotaxis in Bacillus subtilis appears to involve two partially redundant adaptation mechanisms: a receptor methylation-independent process that operates at low attractant concentrations and a receptor methylation-dependent process that is
 
Asparagine chemotaxis in Bacillus subtilis appears to involve two partially redundant adaptation mechanisms: a receptor methylation-independent process that operates at low attractant concentrations and a receptor methylation-dependent process that is
 
required for optimal responses to high concentrations. In order to elucidate these processes, chemotactic responses were assessed for strains
 
required for optimal responses to high concentrations. In order to elucidate these processes, chemotactic responses were assessed for strains
 
expressing methylation-defective mutations in the asparagine receptor, McpB.
 
expressing methylation-defective mutations in the asparagine receptor, McpB.
 +
<br/><br/>
 
<b>Optimization Report</b>
 
<b>Optimization Report</b>
 
<br/><br/>
 
<br/><br/>
Line 24: Line 25:
  
 
<table><tr>
 
<table><tr>
−
<th>[[Image:T--FAFU-CHINA-1mcpb.png|thumb|400px|'''Figure 4 ''':Codon Used Distribution after optimization. ]]</th>
+
<th>[[Image:T--FAFU-CHINA-1mcpb.png|thumb|400px|'''Figure 1 ''':Codon Used Distribution after optimization. ]]</th>
 
</tr></table>
 
</tr></table>
  
 
<br/><br/>
 
<br/><br/>
 
<table><tr>
 
<table><tr>
−
<th>[[Image:T--FAFU-CHINA-2mcpb.png|thumb|400px|'''Figure  5''':GC Content after optimization. ]]</th>
+
<th>[[Image:T--FAFU-CHINA-2mcpb.png|thumb|400px|'''Figure  2''':GC Content after optimization. ]]</th>
 
</tr></table>
 
</tr></table>
  
 
<table><tr>
 
<table><tr>
−
<th>[[Image:T--FAFU-CHINA-dnamcpb.png|thumb|400px|'''Figure 3''': Digested with EcoRI//. ]]</th>
+
<th>[[Image:T--FAFU-CHINA-dnamcpb.png|thumb|400px|'''Figure 3''': Digested with NotI/KpnI. ]]</th>
 +
</tr></table>
 +
 
 +
<br/><br/>
 +
<table><tr>
 +
<th>[[Image:T--FAFU-CHINA--1MCPB.png|thumb|400px|'''Figure  4''':SDS-PAGE of McpB. ]]</th>
 
</tr></table>
 
</tr></table>
  

Latest revision as of 23:11, 1 November 2017


McpB

Chemotaxis is the process by which motile bacteria sense their environment and move towards more favorable surroundings. Environmental ligands are sensed upon binding to transmembrane receptors called methyl-accepting chemotaxis proteins (MCPs).


Usage and Biology

Asparagine chemotaxis in Bacillus subtilis appears to involve two partially redundant adaptation mechanisms: a receptor methylation-independent process that operates at low attractant concentrations and a receptor methylation-dependent process that is required for optimal responses to high concentrations. In order to elucidate these processes, chemotactic responses were assessed for strains expressing methylation-defective mutations in the asparagine receptor, McpB.

Optimization Report

Sequence optimization information:

Expression System: Bacillus megaterium

Gene Length:1986(bp)

Show the relative codon used distribution


Figure 1 :Codon Used Distribution after optimization.



Figure 2:GC Content after optimization.
Figure 3: Digested with NotI/KpnI.



Figure 4:SDS-PAGE of McpB.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]