Difference between revisions of "Part:BBa K2348012"
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<partinfo>BBa_K2348012 short</partinfo><br>
 
<partinfo>BBa_K2348012 short</partinfo><br>
 
This part actually is a composite part containing the alx promoter and riboswitch [https://parts.igem.org/Part:BBa_K2348000 BBa_K2348000] and mNeonGreen [https://parts.igem.org/Part:BBa_K1761003 BBa_K1761003]. But in addition, between riboswitch and mNeonGreen a TEV-site and a f-degron are inserted. Also, mNeonGreen ins 6xHis-taged and the parts ends with a T7-terminator. Because the part does not work when build as Biobrick due to the Biobrick scar this part is listed as basic part.<br>
 
This part actually is a composite part containing the alx promoter and riboswitch [https://parts.igem.org/Part:BBa_K2348000 BBa_K2348000] and mNeonGreen [https://parts.igem.org/Part:BBa_K1761003 BBa_K1761003]. But in addition, between riboswitch and mNeonGreen a TEV-site and a f-degron are inserted. Also, mNeonGreen ins 6xHis-taged and the parts ends with a T7-terminator. Because the part does not work when build as Biobrick due to the Biobrick scar this part is listed as basic part.<br>
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TEV-site and f-degron allow a fast and controllable degradation of mNeonGreen and in combination with the fast maturation time of mNeonGreen allow a fast cooler change when used together with our other pH-sensitive part [https://parts.igem.org/Part:BBa_K2348011 BBa_K2348011]
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TEV-site and F-degron allow a fast and controllable degradation of mNeonGreen wich, in combination with the fast maturation time of mNeonGreen, lead to a fast colour change when used together with our other pH-sensitive part [https://parts.igem.org/Part:BBa_K2348011 BBa_K2348011]
  
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===Usage and Biology===
 
===Usage and Biology===
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The part is used as pH regulated mNeonGreen expression device. Data of expression is shown in the alx Promoter and riboswitch part [https://parts.igem.org/Part:BBa_K2348000 BBa_K2348000].
  
 
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'><b>Sequence and Features</b></span><br>
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<a href="https://static.igem.org/mediawiki/2017/2/27/Alx_part_Graz.jpeg"><img src="Alx_part_Graz.jpeg/799px-Alx_part_Graz.jpegg" alt="Bild" /></a>
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https://static.igem.org/mediawiki/2017/thumb/2/27/Alx_part_Graz.jpeg/799px-Alx_part_Graz.jpeg
 
https://static.igem.org/mediawiki/2017/thumb/2/27/Alx_part_Graz.jpeg/799px-Alx_part_Graz.jpeg
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<partinfo>BBa_K2348012 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2348012 SequenceAndFeatures</partinfo>
  

Latest revision as of 16:54, 1 November 2017


alx promoter and riboswitch + mNeonGreen
This part actually is a composite part containing the alx promoter and riboswitch BBa_K2348000 and mNeonGreen BBa_K1761003. But in addition, between riboswitch and mNeonGreen a TEV-site and a f-degron are inserted. Also, mNeonGreen ins 6xHis-taged and the parts ends with a T7-terminator. Because the part does not work when build as Biobrick due to the Biobrick scar this part is listed as basic part.
TEV-site and F-degron allow a fast and controllable degradation of mNeonGreen wich, in combination with the fast maturation time of mNeonGreen, lead to a fast colour change when used together with our other pH-sensitive part BBa_K2348011

Usage and Biology

The part is used as pH regulated mNeonGreen expression device. Data of expression is shown in the alx Promoter and riboswitch part BBa_K2348000.

Sequence and Features
799px-Alx_part_Graz.jpeg


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 312