Difference between revisions of "Part:BBa K2440010"

 
 
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<partinfo>BBa_K2440010 short</partinfo>
 
<partinfo>BBa_K2440010 short</partinfo>
  
−
Null.
+
This is a dual-luciferase miRNA target expression plasmid.
  
 
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<!-- Add more about the biology of this part here

Latest revision as of 00:01, 2 November 2017


SV40 promoter-> Rluc-> Synthetic PA-> PGK promoter-> Fluc (with BsmBI restriction site)-> SV40 PA

This is a dual-luciferase miRNA target expression plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6049
    Illegal XbaI site found at 4617
    Illegal SpeI site found at 2683
    Illegal PstI site found at 2940
    Illegal PstI site found at 5251
    Illegal PstI site found at 6621
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6049
    Illegal SpeI site found at 2683
    Illegal PstI site found at 2940
    Illegal PstI site found at 5251
    Illegal PstI site found at 6621
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6049
    Illegal BglII site found at 5541
    Illegal BglII site found at 6369
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6049
    Illegal XbaI site found at 4617
    Illegal SpeI site found at 2683
    Illegal PstI site found at 2940
    Illegal PstI site found at 5251
    Illegal PstI site found at 6621
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6049
    Illegal XbaI site found at 4617
    Illegal SpeI site found at 2683
    Illegal PstI site found at 2940
    Illegal PstI site found at 5251
    Illegal PstI site found at 6621
    Illegal NgoMIV site found at 2309
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5730
    Illegal BsaI.rc site found at 1277
    Illegal SapI.rc site found at 2158
    Illegal SapI.rc site found at 2368
    Illegal SapI.rc site found at 3761