Difference between revisions of "Part:BBa K2267041:Design"
(→Source) |
Denise Deng (Talk | contribs) (→Source) |
||
| (One intermediate revision by the same user not shown) | |||
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | + | This part does not include an LVA tag on the LuxR gene. This means that the protein is not rapidly degraded. When active, the LuxR protein binds to the pLux promoter, activating transcription of downstream genes. This part contains everything necessary for O3C6-HSL-induced expression of genes inserted downstream of the pLux promoter. | |
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
| − | + | The parts came from the iGEM 2017 DNA distribution. | |
https://parts.igem.org/Part:BBa_J23119 | https://parts.igem.org/Part:BBa_J23119 | ||
https://parts.igem.org/Part:BBa_C0062 | https://parts.igem.org/Part:BBa_C0062 | ||
Latest revision as of 19:27, 1 November 2017
LuxR-I-Plux-GFP-3
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 837
Illegal NheI site found at 860 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 928
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1452
Illegal BsaI.rc site found at 2179
Design Notes
This part does not include an LVA tag on the LuxR gene. This means that the protein is not rapidly degraded. When active, the LuxR protein binds to the pLux promoter, activating transcription of downstream genes. This part contains everything necessary for O3C6-HSL-induced expression of genes inserted downstream of the pLux promoter.
Source
The parts came from the iGEM 2017 DNA distribution. https://parts.igem.org/Part:BBa_J23119 https://parts.igem.org/Part:BBa_C0062 https://parts.igem.org/Part:BBa_B0034 https://parts.igem.org/Part:BBa_K319039 https://parts.igem.org/Part:BBa_B0010