Difference between revisions of "Part:BBa K2205024"
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<p>This part was made to test the modularity of the system by inserting the P2 Phage sensitivity tuner (BBa_K274371) construct between the two parts of the empty processing module of the Sensynova platform. </p> | <p>This part was made to test the modularity of the system by inserting the P2 Phage sensitivity tuner (BBa_K274371) construct between the two parts of the empty processing module of the Sensynova platform. </p> | ||
| − | <p>The part BBa_K274371 is under the control of our connector 1, part BBa_K2205010, while controlling our connector 2, part BBa_K2205011. </p> | + | <p>The part [https://parts.igem.org/Part:BBa_K274371 BBa_K274371] is under the control of our connector 1, part [https://parts.igem.org/Part:BBa_K2205010 BBa_K2205010], while controlling our connector 2, part [https://parts.igem.org/Part:BBa_K2205011 BBa_K2205011]. </p> |
| − | <p>This system is normally repressed | + | <p>This system is normally repressed except in the presence of connector 1 subsequently activating the transcription of connector 2 (C12 – RHL) triggering the reporter modules of the platform. </p> |
===References=== | ===References=== | ||
Latest revision as of 19:12, 1 November 2017
Sensynova Framework (P2 ogr signal tuner Processing Module - pCell)
Description
The part BBa_K2205024 was designed as Processor unit of the Sensynova multicellular framework for biosensors development (Figure 1).
This part was made to test the modularity of the system by inserting the P2 Phage sensitivity tuner (BBa_K274371) construct between the two parts of the empty processing module of the Sensynova platform.
The part BBa_K274371 is under the control of our connector 1, part BBa_K2205010, while controlling our connector 2, part BBa_K2205011.
This system is normally repressed except in the presence of connector 1 subsequently activating the transcription of connector 2 (C12 – RHL) triggering the reporter modules of the platform.
References
iGEM Community. (2009). Team Cambridge 2009. [online] Available at: http://2009.igem.org/Team:Cambridge [Accessed 30 Oct. 2017].
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 383
Illegal AgeI site found at 580 - 1000COMPATIBLE WITH RFC[1000]