Difference between revisions of "Part:BBa K2505034:Design"

Latest revision as of 18:52, 1 November 2017

traI (Q63G)

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Design Notes

The gene traI(Q63G) is derived from Agrobacterium tumefaciens and encode a enzyme necessary for synthesizing Quorum Sensing signaling molecules ([N-]acyl-homoserine lactones, AHLs), 3OC8 HSL (hereafter C8), in E. coli. This part constitutively produces C8.

The mutation was introduced wild type traI(BBa_K553001).

The DNA sequences of traI (Q63G) is optimized for expressing in E. coli considering the codon usage.

Source

Mutation was newly introduced on the BBa_K553001 by PCR.

References

[1] Pavan Kumar Reddy Kambam, Daniel J. Sayut, Yan Niu, Dawn T. Eriksen, Lianhong Sun (2008) Directed evolution of LuxI for enhanced OHHL production. Biotechnology and Bioengineering Volume 101, Issue 2 1 October 2008 Pages 263-272

[2] MATTHEW R. PARSEK, DALE L. VAL, BRIAN L. HANZELKA, JOHN E. CRONAN, E. P. GREENBERG (1999) Acyl homoserine-lactone quorum-sensing signal generation. Proc. Natl. Acad. Sci. USA Vol. 96, pp. 4360-4365, April 1999 Biochemistry

@@ Line 6: / Line 6: @@
-===Design Notes===
+==Design Notes==
 The gene <i>traI</i>(Q63G) is derived from <i>Agrobacterium tumefaciens</i> and encode a enzyme necessary for synthesizing Quorum Sensing signaling molecules ([N-]acyl-homoserine lactones, AHLs), 3OC8 HSL (hereafter C8), in <i>E. coli</i>. This part constitutively produces C8.
 The mutation was introduced wild type <i>traI</i>(BBa_K553001).
-The DNA sequences of <i>traI</i> (Q63G) is optimized for expressing in <i>E. coli</i> considering the codon usage
+The DNA sequences of <i>traI</i> (Q63G) is optimized for expressing in <i>E. coli</i> considering the codon usage.