Difference between revisions of "Part:BBa K2353000:Experience"
 
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tsPurple was ordered and hydrated from IDT and then digested and ligated into 1C3 so that it could be sent for sequencing and then submitted. Running the digest on a gel showed tsPurple to be the correct length, and sequencing confirmed that the part sequence was correct.
 
tsPurple was ordered and hydrated from IDT and then digested and ligated into 1C3 so that it could be sent for sequencing and then submitted. Running the digest on a gel showed tsPurple to be the correct length, and sequencing confirmed that the part sequence was correct.
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<img src="https://static.igem.org/mediawiki/2017/5/58/Tspurples_gel.jpeg" width="400px">
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<i style="font-size:12px;"> Gel results from a digest of all three tsPurple parts. From top to bottom: BBa_K2353000, BBa_K2353002, BBa_K2353001 </i>
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Using Eurofins for sequencing, we confirmed the parts sequence of tsPurple. The results are shown below, as our tsPurple construct aligned with the tsPurple sequencing results.
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|-<partinfo>BBa_K2353000 Review 1</partinfo>
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<img src="https://static.igem.org/mediawiki/2017/e/e8/Tspurpleimageforregistry.png" width="400px">
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<I>jleveille</I>
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<img src='https://static.igem.org/mediawiki/2017/5/58/Tspurples_gel.jpeg' width='50%'>
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<br><br>
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<i style="font-size:14px;"> Gel results from a digest of all three tsPurple parts. From top to bottom: BBa_K2353000, BBa_K2353002, BBa_K2353001 </i>
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<!-- DON'T DELETE --><partinfo>BBa_K2353000 EndReviews</partinfo>
 
<!-- DON'T DELETE --><partinfo>BBa_K2353000 EndReviews</partinfo>

Latest revision as of 14:44, 1 November 2017


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2353000

User Reviews

UNIQ57645a3c39d598e2-partinfo-00000000-QINU

jleveille

tsPurple was ordered and hydrated from IDT and then digested and ligated into 1C3 so that it could be sent for sequencing and then submitted. Running the digest on a gel showed tsPurple to be the correct length, and sequencing confirmed that the part sequence was correct.
Gel results from a digest of all three tsPurple parts. From top to bottom: BBa_K2353000, BBa_K2353002, BBa_K2353001
Using Eurofins for sequencing, we confirmed the parts sequence of tsPurple. The results are shown below, as our tsPurple construct aligned with the tsPurple sequencing results.


UNIQ57645a3c39d598e2-partinfo-00000004-QINU