Difference between revisions of "Part:BBa K2353000"

Latest revision as of 15:00, 1 November 2017

tsPurple

tsPurple is composed of a ribosomal binding site and tsPurple (a purple chromoprotein which functions as a reporter). tsPurple is intended to be assembled with pLambdaR-LacI (BBa_K1911000),which is the promoter and regulatory system, and pLac-ClpXP-CI (BBa_K1911001),which is the non-lysosomal protease. The terminator is located at the beginning of pLac-ClpXP-CI. When fully assembled, p-lambda-r LacI transcribes tsPurple and LacI represses pLac, preventing transcription of ClpXP-CI. Upon induction with IPTG, LacI binds to IPTG which prevents repression of pLac. Therefore, ClpXP-CI is transcribed; theoretically this should not change the levels of expression of tsPurple since there is no degradation tag attached. The levels of expression of tsPurple with degradation tags can then be compared to the level of tsPurple expression (as a base-line comparison).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2353000 short</partinfo>
-tsPurple is composed of a ribosomal binding site and tsPurple (a purple chromoprotein which functions as a reporter). tsPurple is intended to be assembled with pLambdaR-LacI (which is the promoter and regulatory system) and pLac-ClpXP-CI (which is the non-lysosomal protease). The terminator is located at the beginning of pLac-ClpXP-CI. When fully assembled, p-lambda-r LacI transcribes tsPurple and LacI represses pLac, preventing transcription of ClpXP-CI. Upon induction with IPTG, LacI binds to IPTG which prevents repression of pLac. Therefore, ClpXP-CI is transcribed; theoretically this should not change the levels of expression of tsPurple since there is no degradation tag attached. The levels of expression of tsPurple with degradation tags can then be compared to the level of tsPurple expression (as a base-line comparison).
+tsPurple is composed of a ribosomal binding site and tsPurple (a purple chromoprotein which functions as a reporter). tsPurple is intended to be assembled with pLambdaR-LacI (BBa_K1911000),which is the promoter and regulatory system, and pLac-ClpXP-CI (BBa_K1911001),which is the non-lysosomal protease. The terminator is located at the beginning of pLac-ClpXP-CI. When fully assembled, p-lambda-r LacI transcribes tsPurple and LacI represses pLac, preventing transcription of ClpXP-CI. Upon induction with IPTG, LacI binds to IPTG which prevents repression of pLac. Therefore, ClpXP-CI is transcribed; theoretically this should not change the levels of expression of tsPurple since there is no degradation tag attached. The levels of expression of tsPurple with degradation tags can then be compared to the level of tsPurple expression (as a base-line comparison).
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