Difference between revisions of "Part:BBa K1875003:Experience"

(Characterization by iGEM Kyoto 2017)
(Characterization by iGEM Kyoto 2017)
 
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<img src="https://static.igem.org/mediawiki/parts/8/82/B._xEGFP.jpg" alt="<i>B. xylophilus</i> filled with EGFP in its intestine" style="float:center;width:350px;height:250px;">
 
<img src="https://static.igem.org/mediawiki/parts/8/82/B._xEGFP.jpg" alt="<i>B. xylophilus</i> filled with EGFP in its intestine" style="float:center;width:350px;height:250px;">
 
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Figure1: <i>B. xylophilus</i> full of EGFP in its esophagus.
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Figure1: <i>B. xylophilus</i> full of EGFP in its intestine.
 
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Latest revision as of 02:28, 2 November 2017


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Please enter how you used this part and how it worked out.

Applications of BBa_K1875003

Characterization by [http://2017.igem.org/Team:Kyoto iGEM Kyoto 2017]

We cultured B. xylophilus with S. cerevisiae expressing EGFP of this part from the TDH3 promoter ( BBa_K530008 ). After several days we collected the nematodes and observed them with a fluorescence microscope (fig 1 ), and we were able to confirm nematodes emitting green fluorescence throughout the intestinal tract.

<i>B. xylophilus</i> filled with EGFP in its intestine
Figure1: B. xylophilus full of EGFP in its intestine.

User Reviews

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