Difference between revisions of "Part:BBa K530008"

Latest revision as of 02:18, 2 November 2017

TDH3 Yeast Promoter

TDH3 Promoter is used to regulate the expression of genes within the genome of Saccharomyces cerevisiae or baker's yeast.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Plasmid Map

Sequencing

This is the sequencing for colony 10. Both colony 1 and 10 were a perfect match with the genomic DNA promoter.

CONTRIBUTION

[http://2017.igem.org/Team:Kyoto iGEM Kyoto 2017] improved this part by connecting it with BBa_K1875003(EGFP) , adding further functions. In this improvement, we obtained a new part BBa_K2403003 .

Combination with BBa_K1875003(EGFP)

We combined this part (BBa_K530008, TDH3 promoter) with BBa_K1875003(EGFP) to create the part BBa_K2403003 , a constitutive EGFP expression vector for yeast. We validated the plasmid in S. cerevisiae, and detected a strong green fluorescence under the microscope. ( Please check the figures in experience page of this part. ) In addition, we confirmed that pine wood nematodes eat S. cerevisiae. This innovation greatly contributed to our project.

Revision as of 10:14, 1 November 2017 (view source) TongYou (Talk \| contribs) ← Older edit		Latest revision as of 02:18, 2 November 2017 (view source) TongYou (Talk \| contribs) (→‎CONTRIBUTION)
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	===Combination with BBa_K1875003(EGFP)===		===Combination with BBa_K1875003(EGFP)===
−	We combined this part (BBa_K530008, ~~TDH 3~~ promoter) with ''' [https://parts.igem.org/Part:BBa_K1875003 BBa_K1875003(EGFP)] ''' to create the part ''' [https://parts.igem.org/Part:BBa_K2403003 BBa_K2403003] ''', a constitutive EGFP expression vector for yeast. We validated the plasmid in <i>S. cerevisiae,</i> and detected a strong green fluorescence under the microscope. (~~The figure is on~~ '''[]'''.) In addition, we confirmed that pine wood nematodes eat <i>S. cerevisiae</i>. This innovation greatly contributed to our project.	+	We combined this part (BBa_K530008, TDH3 promoter) with ''' [https://parts.igem.org/Part:BBa_K1875003 BBa_K1875003(EGFP)] ''' to create the part ''' [https://parts.igem.org/Part:BBa_K2403003 BBa_K2403003] ''', a constitutive EGFP expression vector for yeast. We validated the plasmid in <i>S. cerevisiae,</i> and detected a strong green fluorescence under the microscope. ( Please check the figures in '''[https://parts.igem.org/Part:BBa_K530008:Experience experience page of this part]'''. ) In addition, we confirmed that pine wood nematodes eat <i>S. cerevisiae</i>. This innovation greatly contributed to our project.