Difference between revisions of "Part:BBa K2462003"

Latest revision as of 15:29, 1 November 2017

Promoter for Bacillus megaterium

Description

This part is a promoter used in Bacillus megaterium. We got its sequence in pSPAsp-hp, which is a plasmid used for protein expression in Bacillus megaterium. Then the whole part has been synthesized by AuGCT.

Verification

As for the verification of this promoter, we combined it with BBa_K2462001 and BBa_K2462000 in order to make a circuit. We planned to test this promoter through the test of our enzyme's effect. Several experiments have been done,and details can be found in the following two pages:

https://parts.igem.org/Part:BBa_K2462001

https://parts.igem.org/wiki/index.php?title=Part:BBa_K2462000

The results of experiments are depicted as following:

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Disscusion

This year, our team decided to carry on a project using Bacillus megaterium as the host. Through our own experience, we felt that there's few parts in the past years related to the expression in Bacillus mageterium although it's a great host for many protein to be expressed. Thus, when making parts, we considered more about filling this blank and give the following teams convenience to use Bacillus megaterium. Unfortunately, we are not able to verify this part successfully, but we do welcome other teams to use our part and hope our parts can bring them help. As this promoter was from pSPAsp-hp——a plasmid in Addgene, few articles using this promoter can be found in their page for other teams’ reference.

More details about pSPAsp-hp:

http://www.addgene.org/48122/

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K2462003 short</partinfo>
+===Description===
 This part is a promoter used in Bacillus megaterium. We got its sequence in pSPAsp-hp, which is a plasmid used for protein expression in Bacillus megaterium. Then the whole part has been synthesized by AuGCT.
-<!-- Add more about the biology of this part here
+===Verification===
-===Usage and Biology===
+As for the verification of this promoter, we combined it with BBa_K2462001 and BBa_K2462000 in order to make a circuit. We planned to test this promoter through the test of our enzyme's effect. Several experiments have been done,and details can be found in the following two pages:
+https://parts.igem.org/Part:BBa_K2462001
+https://parts.igem.org/wiki/index.php?title=Part:BBa_K2462000
+The results of experiments are depicted as following:
+<!-- Add more about the biology of this part here
 <!-- -->
 <br>
@@ Line 14: / Line 22: @@
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K2462003 SequenceAndFeatures</partinfo>
+===Disscusion===
+This year, our team decided to carry on a project using Bacillus megaterium as the host. Through our own experience, we felt that there's few parts in the past years related to the expression in Bacillus mageterium although it's a great host for many protein to be expressed. Thus, when making parts, we considered more about filling this blank and give the following teams convenience to use Bacillus megaterium. Unfortunately, we are not able to verify this part successfully, but we do welcome other teams to use our part and hope our parts can bring them help. As this promoter was from pSPAsp-hp——a plasmid in Addgene, few articles using this promoter can be found in their page for other teams’ reference.
+More details about pSPAsp-hp:
+http://www.addgene.org/48122/
 <!-- Uncomment this to enable Functional Parameter display