Difference between revisions of "Part:BBa K2365049"
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[[File: Pdc1 pdc1-cyc1.jpeg|500px|center]] | [[File: Pdc1 pdc1-cyc1.jpeg|500px|center]] | ||
| − | We inserted GPF (S6GT) as the index of promoter test. After 30 hours incubation, we measured the fluorescence intensity of the transformed yeast at 440 nm to 530 nm | + | We used reserved restriction enzyme cutting site to inserted GPF (S6GT) between promoter and termiator as the index of promoter test. After 30 hours incubation, we measured the fluorescence intensity of the transformed yeast at 440 nm to 530 nm |
[[File: U-disk test.jpg|500px|center]] | [[File: U-disk test.jpg|500px|center]] | ||
[[File:酵母荧光.jpg|700px|center]] | [[File:酵母荧光.jpg|700px|center]] | ||
Latest revision as of 09:16, 1 November 2017
PDC1 promoter-CYC1 terminator
Between the PDC1 promoter and cyc1 terminator,having the restriction enzyme cutting site.And you can insert the gene if you want.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 289
We used reserved restriction enzyme cutting site to inserted GPF (S6GT) between promoter and termiator as the index of promoter test. After 30 hours incubation, we measured the fluorescence intensity of the transformed yeast at 440 nm to 530 nm
