Difference between revisions of "Part:BBa K2365031"

 
(4 intermediate revisions by 2 users not shown)
Line 18: Line 18:
 
<partinfo>BBa_K2365031 parameters</partinfo>
 
<partinfo>BBa_K2365031 parameters</partinfo>
 
<!-- -->
 
<!-- -->
PCR result
+
====PCR result====
 
[[File:TDH1 TDH1-cyc1 NAU-04.jpeg|300px|center]]
 
[[File:TDH1 TDH1-cyc1 NAU-04.jpeg|300px|center]]
[[File:酵母荧光.jpg|500px|center]]
+
[[File:启动子全部.jpeg|700px|center]]
 +
Figure.The fluorescence of the four test devices and control transformed into Saccharomyces cerevisiae and inoculated in YPD broth was measured after 8h. 
 +
Nevertheless, the attractiveness of yeast as a platform organism (especially for metabolic engineering applications) needs continued efforts to expand the set of tools available.

Latest revision as of 01:25, 2 November 2017


TDH1 promotor

TDH1 Yeast promoter Constitutive expression ; Glyceraldehyde-3-phosphate dehydrogenase,a weak constitutive promoter,which catalyzed 3-phosphoglyceraldehyde oxidation (dehydrogenation) and phosphorylation.As the center of glucose metabolism,TDH1 plays an important role in glucose metabolism. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 431
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


PCR result

TDH1 TDH1-cyc1 NAU-04.jpeg
启动子全部.jpeg

Figure.The fluorescence of the four test devices and control transformed into Saccharomyces cerevisiae and inoculated in YPD broth was measured after 8h. Nevertheless, the attractiveness of yeast as a platform organism (especially for metabolic engineering applications) needs continued efforts to expand the set of tools available.