Difference between revisions of "Part:BBa K2433004:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | The | + | The <i>virB1</i> promoter was designed by taking the promoter region of <i>virB1</i> from the pTiC58 - a Ti plasmid from <i>Agrobacterium tumefaciens</i>. The nucleotide at position 339 was changed to a T to eliminate an XbaI cutsite. |
===Source=== | ===Source=== |
Latest revision as of 03:30, 2 November 2017
VirB1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 118
Design Notes
The virB1 promoter was designed by taking the promoter region of virB1 from the pTiC58 - a Ti plasmid from Agrobacterium tumefaciens. The nucleotide at position 339 was changed to a T to eliminate an XbaI cutsite.
Source
Upstream regulatory region of virB1 from pTiC58.
References
AE007871.2