Difference between revisions of "Part:BBa K2281006"
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SIZE 1455 bp | SIZE 1455 bp | ||
− | DEFINITION Hevea brasiliensis 12-oxophytodienoate reductase (opr) | + | DEFINITION Hevea brasiliensis 12-oxophytodienoate reductase (opr) |
+ | |||
HbOYE stands for Hevea brasiliensis Old Yellow Enzyme which is the reductase which bolsters the production of cireonellol from geraniol. | HbOYE stands for Hevea brasiliensis Old Yellow Enzyme which is the reductase which bolsters the production of cireonellol from geraniol. | ||
− | + | [[File:Mosquito_parts-figure1.png|center|940px|img]] | |
The diagram shows the complete pathway of the production of citronellol and OYE is emplyed in the final step of the process. | The diagram shows the complete pathway of the production of citronellol and OYE is emplyed in the final step of the process. | ||
− | + | The sequence of forward primer (NEB-HbOYE-F-EcoRI) is: CGGAATTCATGGCTGAAACTGGAACAG | |
− | + | ||
− | The sequence of forward primer (NEB-HbOYE-F-EcoRI) is: | + | |
− | + | ||
− | + | ||
− | + | The sequence of reverse primer (NEB-HbOYE1-R-PstI) is: AACTGCAGTCAAAGGCGTGATCGTGGCT | |
===Sources=== | ===Sources=== |
Latest revision as of 04:00, 2 November 2017
--HbOYE--
Introduction
LOCUS DQ004685
SIZE 1455 bp
DEFINITION Hevea brasiliensis 12-oxophytodienoate reductase (opr)
HbOYE stands for Hevea brasiliensis Old Yellow Enzyme which is the reductase which bolsters the production of cireonellol from geraniol.
The diagram shows the complete pathway of the production of citronellol and OYE is emplyed in the final step of the process.
The sequence of forward primer (NEB-HbOYE-F-EcoRI) is: CGGAATTCATGGCTGAAACTGGAACAG
The sequence of reverse primer (NEB-HbOYE1-R-PstI) is: AACTGCAGTCAAAGGCGTGATCGTGGCT
Sources
SOURCE Hevea brasiliensis
Hevea brasiliensis, the Pará rubber tree, sharinga tree, seringueira, or, most commonly, the rubber tree or rubber plant, is a tree belonging to the family Euphorbiaceae. It is the most economically important member of the genus Hevea because the milky latex extracted from the tree is the primary source of natural rubber.
ORGANISM Hevea brasiliensis Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Malpighiales; Euphorbiaceae; Crotonoideae; Micrandreae; Hevea.
H. brasiliensis is a tall deciduous tree growing to a height of up to 40 m (131 ft) in the wild, but cultivated trees are usually much smaller because drawing off the latex restricts the growth of the tree. The trunk is cylindrical and may have a swollen, bottle-shaped base. The bark is some shade of brown, and the inner bark oozes latex when damaged. The leaves have three leaflets and are spirally arranged. The inflorescence include separate male and female flowers. The flowers are pungent, creamy-yellow and have no petals. The fruit is a capsule that contains three large seeds; it opens explosively when ripe.
References
REFERENCE 1 (bases 1 to 1455)
AUTHORS Norton,G., Pappusamy,A., Yusof,F., Pujade-Renaud,V., Perkins,M., Griffiths,D. and Jones,H. TITLE Characterisation of recombinant Hevea brasiliensis allene oxide synthase: effects of cycloxygenase inhibitors, lipoxygenase inhibitors and salicylates on enzyme activity JOURNAL Plant Physiol. Biochem. 45 (2), 129-138 (2007) PUBMED 17344058
REFERENCE 2 (bases 1 to 1455)
AUTHORS Norton,G., Arokiaraj,P., Yusof,F., Pujade-Renaud,V., Griffiths,D. and Jones,H. TITLE Direct Submission JOURNAL Submitted (12-APR-2005) Division of Biosciences, University of Hertfordshire, College Lane, Hatfield, Herts AL10 9AB, UK
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]