Difference between revisions of "Part:BBa K2271010"

Latest revision as of 03:38, 2 November 2017

Pex34p with ScCCW12 promoter

This part contains the coding sequence for the peroxin Pex34 with the strong constitutive promoter ScSSW12 and the terminator ScADH1. The sequence is codon-optimized for S. cerevisiae.

Usage and Biology

Pex34p is a peroxisomal integral membrane protein. It can act independently as well as in combination with Pex11p, Pex25p, and Pex27p to control the peroxisome morphology and population. Pex34p is suggested to directly influence peroxisome proliferation as well as constitutive peroxisome division. Overexpression of Pex34 leeds to a high number of large peroxisomes.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal suffix found in sequence at 1139
Illegal BglII site found at 701
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 18:02, 31 October 2017 (view source) Pauline tot (Talk \| contribs) ← Older edit		Latest revision as of 03:38, 2 November 2017 (view source) Anna Sappler (Talk \| contribs)
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	===Usage and Biology===		===Usage and Biology===
−	Pex34p is a peroxisomal integral membrane protein ~~that~~ can act ~~both,~~ independently ~~and~~ in combination with Pex11p, Pex25p, and Pex27p to control the peroxisome morphology and population. Pex34p is suggested to directly influence peroxisome proliferation as well as constitutive peroxisome division.	+	Pex34p is a peroxisomal integral membrane protein. It can act independently as well as in combination with Pex11p, Pex25p, and Pex27p to control the peroxisome morphology and population. Pex34p is suggested to directly influence peroxisome proliferation as well as constitutive peroxisome division. Overexpression of Pex34 leeds to a high number of large peroxisomes.