Difference between revisions of "Part:BBa K2365034"
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We added zearalenone by 1ug/ml and the degrading enzyme start to work.the ZEN is degraded as time goes by.The obvious degrading effect is proved by the height of Uv mapping | We added zearalenone by 1ug/ml and the degrading enzyme start to work.the ZEN is degraded as time goes by.The obvious degrading effect is proved by the height of Uv mapping | ||
| − | [[Image:Uv.png|thumb|center|500px|High Performance Liquid Chromatography(HPLC) analysis for acetylation ability of ZHD101]] | + | [[Image:Uv.png|thumb|center|500px|Fig3 shows High Performance Liquid Chromatography(HPLC) analysis for acetylation ability of ZHD101]] |
We use the High Performance Liquid Chromatography(HPLC) to analyze the ability of acetylation of ZHD101.The blue color means the effect after two hour,black means four hour effect,pink means 8 hour,dark red means 12hour effect. | We use the High Performance Liquid Chromatography(HPLC) to analyze the ability of acetylation of ZHD101.The blue color means the effect after two hour,black means four hour effect,pink means 8 hour,dark red means 12hour effect. | ||
Latest revision as of 16:13, 31 October 2017
ZHD101--Zearalenone degrading enzyme
In our developed assay, the gene encoding ZHD101（a ZEN-degrading enzyme）was cloned. ZHD101, a lactonohydrolase produced by the fungal species Clonostachys rosea, converts ZEN into 1-(3,5-dihydroxy-phenyl)-10-hydroxy-1-undecen- 6-one, which is a markedly less toxic product. In previous studies, ZHD101 has been shown to be successfully expressed by Escherichia coli, Saccharomyces cerevisiae, and rice plants, and the recombinant ZHD101 produced by these genetically modifed organisms efectively degraded ZEN .
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 772
PCR Result
This is our Uv mapping result,for this result we set four time point ,Z2 as our time point 1 which are taken after 2 hours’culturation ,shown in pink colour line on the figure and Z4 as time point 2 which are taken after 4 hours’culturation,shown in dark blue line while Z6 as our time point 3 which are taken after 6 hours’culturation shown in pretty blue line. the bottom of the figure is the point 4 which are taken after 8 hours’culturation. We added zearalenone by 1ug/ml and the degrading enzyme start to work.the ZEN is degraded as time goes by.The obvious degrading effect is proved by the height of Uv mapping
We use the High Performance Liquid Chromatography(HPLC) to analyze the ability of acetylation of ZHD101.The blue color means the effect after two hour,black means four hour effect,pink means 8 hour,dark red means 12hour effect.