Difference between revisions of "Part:BBa K2273013:Design"
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===Source=== | ===Source=== | ||
| − | The <i>comK</i> | + | The <i>comK</i> promoter was amplified via PCR from the <i>B. subtilis</i> wild type W168 genome using the primers listed below. |
<br> | <br> | ||
<table> | <table> | ||
Latest revision as of 16:04, 31 October 2017
PcomK (mutated): a ComK-dependent promoter
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design
This part was submitted with RFC10 Suffix and Prefix. It encodes for the mutated (mut) comK promoter that is part of the ComX quorum response of B. subtilis and was designed for the subproject "Communication" by the [http://2017.igem.org/Team:TU_Dresden/Project/Communication iGEM Team TU Dresden 2017 (EncaBcillus - It's a trap!)]. In order to remove a SpeI restriction site one nucleotide was exchanged (nt 32, A to T). Therefore it is marked as mutated (mut).
Source
The comK promoter was amplified via PCR from the B. subtilis wild type W168 genome using the primers listed below.
| PcomK mut fwd RFC10 | gatcTCTAGAGCAAGTCTTATGAAAGTAAAATCGGTTTATTTCTAG |
This primer only contains the XbaI restriction site of the prefix. The nucleotide marked bold was exchanged (A to T) in order to remove a SpeI restriction site. |
| PcomK mut rev RFC10 | gatcCTGCAGCGGCCGCTACTAGTTTTTCTGCAAAATTTATACTAATAATC |
References
Susanna KA, van der Werff AF, den Hengst CD, Calles B, Salas M, Venema G, Hamoen LW, Kuipers OP.(2014) Mechanism of transcription activation at the comG promoter by the competence transcription factor ComK of Bacillus subtilis. J Bacteriol. 186(4):1120-8 PubMed
Hamoen LW, Venema G, Kuipers OP (2003) Controlling competence in Bacillus subtilis: shared use of regulators. Microbiology. 149(Pt 1):9-17 PubMed