Difference between revisions of "Part:BBa K2365035"
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[[File:ZHD101 DNA.jpg|400px]] | [[File:ZHD101 DNA.jpg|400px]] | ||
[[File:ZHD101 SDS.jpg|400px]] | [[File:ZHD101 SDS.jpg|400px]] | ||
| + | ====High Performance Liquid Chromatography(HPLC) analysis==== | ||
| + | [[File:Tupian2.jpg|thumb|center|500px|Fig3 shows that We use the High Performance Liquid Chromatography(HPLC) to analyze the abi lity of zearalenone(ZEN) degrading enzyme.]] | ||
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Figure3: from articles we know that the absorption peak is about in 245 nm. The figure above shows :Data 1 represents the sample only with ZEN as contrast group ,the peak appears at 2.5 min represents ZEN and the peaks appear at 3.5min represent the isomers of ZEN.its peak area is about 27570 uV·min. The others(data2,3,4) represtents our test groups.compared with the contrast group,we preliminarily proved ZEN-degrading enzyme from the sequence (which come from GenBank AB076037 and then optimized by us) works from the discreasing peak area. | Figure3: from articles we know that the absorption peak is about in 245 nm. The figure above shows :Data 1 represents the sample only with ZEN as contrast group ,the peak appears at 2.5 min represents ZEN and the peaks appear at 3.5min represent the isomers of ZEN.its peak area is about 27570 uV·min. The others(data2,3,4) represtents our test groups.compared with the contrast group,we preliminarily proved ZEN-degrading enzyme from the sequence (which come from GenBank AB076037 and then optimized by us) works from the discreasing peak area. | ||
| + | ====Third experiment==== | ||
| + | [[File:Zenjiang.jpeg|600px|center]] | ||
| + | And the ability of degradation of ZEN can reach 100% at 14h. | ||
Latest revision as of 21:56, 1 November 2017
ZHD101--Zearalenone degrading enzyme (optimized)
the gene encoding ZHD101（a ZEN-degrading enzyme）was cloned. ZHD101, a lactonohydrolase produced by the fungal species Clonostachys rosea, converts ZEN into 1-(3,5-dihydroxy-phenyl)-10-hydroxy-1-undecen- 6-one, which is a markedly less toxic product.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
PCR and SDS-PAGE
Showing the size of the target gene and the protein.
High Performance Liquid Chromatography(HPLC) analysis
Figure3: from articles we know that the absorption peak is about in 245 nm. The figure above shows :Data 1 represents the sample only with ZEN as contrast group ,the peak appears at 2.5 min represents ZEN and the peaks appear at 3.5min represent the isomers of ZEN.its peak area is about 27570 uV·min. The others(data2,3,4) represtents our test groups.compared with the contrast group,we preliminarily proved ZEN-degrading enzyme from the sequence (which come from GenBank AB076037 and then optimized by us) works from the discreasing peak area.
Third experiment
And the ability of degradation of ZEN can reach 100% at 14h.