Difference between revisions of "Part:BBa I742144"

Latest revision as of 18:34, 25 October 2007

Plac+lacZ+sam8 (tyrosine ammonia lyase)

This part consists of sam8, encoding tyrosine ammonia lyase (converts tyrosine to p-coumaric acid) from the bacterium Saccharothrix espanaensis DSM 44229, inserted downstream of a lac promoter with attached lacZ' minigene. The purpose of this was to ensure sam8 expression on induction with IPTG and also to provide a visually selectable tag when assembling the entire pathway; by always inserting a lacZ-containing part into a non-lacZ-containing part it is easy to select the correct clones on the basis that they form blue colonies.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1416
Illegal BamHI site found at 1818
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1159
Illegal AgeI site found at 1106
Illegal AgeI site found at 1275
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 710
Illegal BsaI site found at 976

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 <partinfo>BBa_I742144 short</partinfo>
-This part consists of sam8, encoding tyrosine ammonia lyase (converts tyrosine to p-coumaric acid) from the bacterium ''Saccharothrix espanaensis'' DSM 44229, inserted downstream of a ''lac'' promoter with attached ''lacZ''' minigene. The purpose of this was to ensure sam8 expression on induction with IPTG and also to provide a visually selectable tag when assembling the entire pathway; by always inserting a ''lacZ'''-containing part into a non-''lacZ'''-containing part it is easy to select the correct clones on the basis that they form blue colonies.
+This part consists of sam8, encoding tyrosine ammonia lyase (converts tyrosine to p-coumaric acid) from the bacterium ''Saccharothrix espanaensis'' DSM 44229, inserted downstream of a ''lac'' promoter with attached ''lacZ''' minigene. The purpose of this was to ensure sam8 expression on induction with IPTG and also to provide a visually selectable tag when assembling the entire pathway; by always inserting a ''lacZ''-containing part into a non-''lacZ''-containing part it is easy to select the correct clones on the basis that they form blue colonies.
 <!-- Add more about the biology of this part here