Difference between revisions of "Part:BBa K530008"
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<partinfo>BBa_K530008 short</partinfo>
 
<partinfo>BBa_K530008 short</partinfo>
  
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TDH3 Promoter is used to regulate the expression of genes within the genome of Saccharomyces Cerevisiae or baker's yeast.
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TDH3 Promoter is used to regulate the expression of genes within the genome of <i>Saccharomyces cerevisiae</i> or baker's yeast.
  
 
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==<big>''' CONTRIBUTION '''</big>==
 
==<big>''' CONTRIBUTION '''</big>==
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''' [http://2017.igem.org/Team:Kyoto  iGEM Kyoto 2017] ''' <big>improved</big> this part by connecting this part with ''' [https://parts.igem.org/Part:BBa_K1875003  BBa_K1875003(EGFP)] ''' and added further functions. In this improvement, we obtained a new part ''' [https://parts.igem.org/Part:BBa_K2403003  BBa_K2403003] '''.
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''' [http://2017.igem.org/Team:Kyoto  iGEM Kyoto 2017] ''' <big>improved</big> this part by connecting it with ''' [https://parts.igem.org/Part:BBa_K1875003  BBa_K1875003(EGFP)] ''', adding further functions. In this improvement, we obtained a new part ''' [https://parts.igem.org/Part:BBa_K2403003  BBa_K2403003] '''.
  
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===Combination with BBa_K530008 (TDH3 promoter)===
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===Combination with BBa_K1875003(EGFP)===
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With this combination, this part BBa_K530008 (TDH 3 promoter) was used to create the part BBa_K2403003 which codes EGFP emitting strong green fluorescence. As a result, it was possible to confirm yeast emitting a strong green fluorescence under the microscope (photo or link). In addition, we confirmed that the pine wood nematode eats S. cerevisiae. Such usage is innovative and has greatly contributed to our project.
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We combined this part (BBa_K530008, TDH3 promoter) with ''' [https://parts.igem.org/Part:BBa_K1875003  BBa_K1875003(EGFP)] ''' to create the part ''' [https://parts.igem.org/Part:BBa_K2403003 BBa_K2403003] ''', a constitutive EGFP expression vector for yeast. We validated the plasmid in <i>S. cerevisiae,</i> and detected a strong green fluorescence under the microscope. ( Please check the figures in '''[https://parts.igem.org/Part:BBa_K530008:Experience experience page of this part]'''. ) In addition, we confirmed that pine wood nematodes eat <i>S. cerevisiae</i>. This innovation greatly contributed to our project.

Latest revision as of 02:18, 2 November 2017

TDH3 Yeast Promoter

TDH3 Promoter is used to regulate the expression of genes within the genome of Saccharomyces cerevisiae or baker's yeast.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Plasmid Map

TDH3p Plasmid Map.png


Sequencing

TDH3p Sequencing.png


This is the sequencing for colony 10. Both colony 1 and 10 were a perfect match with the genomic DNA promoter.


CONTRIBUTION

[http://2017.igem.org/Team:Kyoto iGEM Kyoto 2017] improved this part by connecting it with BBa_K1875003(EGFP) , adding further functions. In this improvement, we obtained a new part BBa_K2403003 .

Combination with BBa_K1875003(EGFP)

We combined this part (BBa_K530008, TDH3 promoter) with BBa_K1875003(EGFP) to create the part BBa_K2403003 , a constitutive EGFP expression vector for yeast. We validated the plasmid in S. cerevisiae, and detected a strong green fluorescence under the microscope. ( Please check the figures in experience page of this part. ) In addition, we confirmed that pine wood nematodes eat S. cerevisiae. This innovation greatly contributed to our project.