Difference between revisions of "Part:BBa K2377005"

Latest revision as of 22:50, 31 October 2017

TrxzL, a developed element to generate trxz hpRNA with loop120

We developed trxzL to produce trxz hpRNA with loop120. We found that the hairpin RNA actually possess higher stability and higher efficiency in gene silencing in living organisms including higher plants like Arabidopsis thaliana. In general, the hairpin RNA harbor similar effect with double-stranded RNA, what is different is that there is a loop inserted between the sense arm and anti-sense arm.

TRX z is a branch of plastidial thioredoxin (TRX), lying between x- and y-type TRXs thus was named TRX z, which is a likely candidate for mediating thiol-modifications in chloroplasts. Recently, several independent studies could demonstrate that TRX z constitutes a subunit of the plastid-encoded RNA polymerase (PEP). Meanwhile the generation of the full PEP complement is an essential early step during chloroplast bio-genesis and its disturbance cannot be reversed if a certain time point in the program has been passed. The Arabidopsis knockout mutant lines of TRX z exhibit a complete albino phenotype and was inhibited in chloroplast development.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 284
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2377005 short</partinfo>
-A developed element to generate trxz hpRNA with a normal loop, We found that the hairpin RNA actually possess higher stability and higher efficiency in gene silencing in living organisms including higher plants like Arabidopsis thaliana. In general, the hairpin RNA harbor similar effect with double-stranded RNA, what is different is that there is a loop inserted between the sense arm and anti-sense arm.
+We developed trxzL to produce trxz hpRNA with loop120.  We found that the hairpin RNA actually possess higher stability and higher efficiency in gene silencing in living organisms including higher plants like Arabidopsis thaliana. In general, the hairpin RNA harbor similar effect with double-stranded RNA, what is different is that there is a loop inserted between the sense arm and anti-sense arm.
 https://static.igem.org/mediawiki/2017/4/48/Lanzhou_wiki_Design_figure2.png
+TRX z is a branch of plastidial thioredoxin (TRX), lying between x- and y-type TRXs thus was named TRX z, which is a likely candidate for mediating thiol-modifications in chloroplasts. Recently, several independent studies could demonstrate that TRX z constitutes a subunit of the plastid-encoded RNA polymerase (PEP). Meanwhile the generation of the full PEP complement is an essential early step during chloroplast bio-genesis and its disturbance cannot be reversed if a certain time point in the program has been passed. The Arabidopsis knockout mutant lines of TRX z exhibit a complete albino phenotype and was inhibited in chloroplast development.
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