Difference between revisions of "Part:BBa I732106"
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<partinfo>BBa_I732106 short</partinfo> | <partinfo>BBa_I732106 short</partinfo> | ||
− | [[Image:USTC_allen2.jpg|thumb|right|150px|'''Figure I''' The recognition region of Lac repressor of which the amino acid residues we will try to modify. This figure comes from 'Roberto Kopke Salinas, | + | [[Image:USTC_allen2.jpg|thumb|right|150px|'''Figure I''' The recognition region of Lac repressor of which the amino acid residues we will try to modify. This figure comes from 'Roberto Kopke Salinas, et al. ''ChemBioChem 2005, 6, 1628 - 1637'']] |
− | The artificial repressor comes from the results by [http:// | + | The artificial repressor comes from the results by [http://2007.igem.org/USTC/Repressor_Evolution_on_Plates Repressor Evolution on Plates]. The natural lactose repressor protein has been redesigned at Position 17, 18 of a.a. sequence, which is the composition of amino acid of recognition helix (Figure I). Standard BioBrick Prefix and Suffix are added to both ends of this part for standard assembly. |
===Usage and Biology=== | ===Usage and Biology=== | ||
− | *This artificial lac-repressor can bind to the specific promoters with high affinity while weakly bind to other promoters with relatively low affinity. The repression intensity is indicated in the Repression Matrix shown as below. How to acquire this repressor and how to obtain Repressor-Promoter pairs will been shown in [http:// | + | *This artificial lac-repressor can bind to the specific promoters with high affinity while weakly bind to other promoters with relatively low affinity. The repression intensity is indicated in the Repression Matrix shown as below. How to acquire this repressor and how to obtain Repressor-Promoter pairs will been shown in [http://2007.igem.org/USTC/Repressor_Evolution_on_Plates This Page]. |
*This repressor sequence is single repressor expression parts without other functional gene parts such as RBS & Terminators. The same repressor sequence with RBS can be found in [https://parts.igem.org/wiki/index.php/Part:BBa_I732806 BBa_I732806] as a Standard BioBrick part. The same repressor sequence with both RBS and Terminator can be found in [https://parts.igem.org/wiki/index.php/Part:BBa_I732826 BBa_I732826] as a Standard BioBrick part. | *This repressor sequence is single repressor expression parts without other functional gene parts such as RBS & Terminators. The same repressor sequence with RBS can be found in [https://parts.igem.org/wiki/index.php/Part:BBa_I732806 BBa_I732806] as a Standard BioBrick part. The same repressor sequence with both RBS and Terminator can be found in [https://parts.igem.org/wiki/index.php/Part:BBa_I732826 BBa_I732826] as a Standard BioBrick part. |
Latest revision as of 18:11, 25 October 2007
ARL2A0102
The artificial repressor comes from the results by [http://2007.igem.org/USTC/Repressor_Evolution_on_Plates Repressor Evolution on Plates]. The natural lactose repressor protein has been redesigned at Position 17, 18 of a.a. sequence, which is the composition of amino acid of recognition helix (Figure I). Standard BioBrick Prefix and Suffix are added to both ends of this part for standard assembly.
Usage and Biology
- This artificial lac-repressor can bind to the specific promoters with high affinity while weakly bind to other promoters with relatively low affinity. The repression intensity is indicated in the Repression Matrix shown as below. How to acquire this repressor and how to obtain Repressor-Promoter pairs will been shown in [http://2007.igem.org/USTC/Repressor_Evolution_on_Plates This Page].
- This repressor sequence is single repressor expression parts without other functional gene parts such as RBS & Terminators. The same repressor sequence with RBS can be found in BBa_I732806 as a Standard BioBrick part. The same repressor sequence with both RBS and Terminator can be found in BBa_I732826 as a Standard BioBrick part.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Unknown
- 1000COMPATIBLE WITH RFC[1000]