Difference between revisions of "Part:BBa K2404016"
 
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__NOTOC__
 
__NOTOC__
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<partinfo>BBa_K2404016 short</partinfo>
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<partinfo>BBa_K2404016 short</partinfo><br>
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We generated a luciferase construct to test the efficacy of the tobacco leave expression system.
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This construct contains the [https://parts.igem.org/Part:BBa_P10000 LexA promotor], the luciferase coding sequence and the [https://parts.igem.org/Part:BBa_P10401 Nos terminator].  
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This construct contains the [https://parts.igem.org/Part:BBa_P10000 LexA promotor], the [https://parts.igem.org/Part:BBa_K2404001 TSHH] coding sequence and the [https://parts.igem.org/Part:BBa_P10401 Nos terminator].  
  
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This part is contained within the Phytobrick level 1 [https://parts.igem.org/Part:BBa_P10501 pGB-A1 plasmid].
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This part is contained within the Phytobrick level 1 [https://parts.igem.org/Part:BBa_P10503 pGB-A2 plasmid].
 
<br>
 
<br>
 
The LexA promoter is induced by the XVE protein in the presence of estradiol so to be active requires co-expression with the XVE protein.  
 
The LexA promoter is induced by the XVE protein in the presence of estradiol so to be active requires co-expression with the XVE protein.  
  
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A thyroid stimulating hormone antagonist, with His-Tags for purification, under the control of the LexA XVE-inducible promoter native to <i> E. coli </i>. This has the Nopaline synthase gene terminator from <i> Agrobacterium tumefaciens </i>. The construct is contained within the pGB-A2 plasmid. It should be used to produce the TSH antagonist under control of XVE.
 
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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Sequence details:
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<br>
 
1-6: BsmBI:<br>
 
1-6: BsmBI:<br>
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7-348: LexA:<br>
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12-356: LexA<br>
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349-2005: LUC+:<br>
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357-1169: TSHH<br>
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2006-2272: NosT:<br>
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1174-1436: NosT<br>
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2282-2287: BsmBI:<br>
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1456-1461: BsmBI<br>
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<partinfo>BBa_K2404016 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2404016 SequenceAndFeatures</partinfo>

Latest revision as of 09:46, 31 October 2017


A TSH antagonist with His-Tags, controlled by the LexA promoter

This construct contains the LexA promotor, the TSHH coding sequence and the Nos terminator.

This part is contained within the Phytobrick level 1 pGB-A2 plasmid.
The LexA promoter is induced by the XVE protein in the presence of estradiol so to be active requires co-expression with the XVE protein.


Sequence and Features Sequence details:
1-6: BsmBI:
12-356: LexA
357-1169: TSHH
1174-1436: NosT
1456-1461: BsmBI



Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 22
    Illegal PstI site found at 888
    Illegal PstI site found at 930
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 22
    Illegal PstI site found at 888
    Illegal PstI site found at 930
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1143
    Illegal XhoI site found at 955
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 22
    Illegal PstI site found at 888
    Illegal PstI site found at 930
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 22
    Illegal PstI site found at 888
    Illegal PstI site found at 930
  • 1000
    COMPATIBLE WITH RFC[1000]