Difference between revisions of "Part:BBa K2276007:Design"
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We want to engineer a strain which can produce melatonin at daily rhythm.We employ repressilator system thus allowing the E.coli coding enzyme periodically. However, the cycle time is not 24h.We built a model to analyse the issue.According to the medeling result, a stronger RBS was needed.We turned to the bioinformatic methods to design a RBS predicted to show hign translation rate.This is the website on which we performed the design: https://salislab.net/software/doForwardRBS. We designed three, and BBa_K2276007 is one of them. | We want to engineer a strain which can produce melatonin at daily rhythm.We employ repressilator system thus allowing the E.coli coding enzyme periodically. However, the cycle time is not 24h.We built a model to analyse the issue.According to the medeling result, a stronger RBS was needed.We turned to the bioinformatic methods to design a RBS predicted to show hign translation rate.This is the website on which we performed the design: https://salislab.net/software/doForwardRBS. We designed three, and BBa_K2276007 is one of them. | ||
− | [[File:P1.png|1200px|thumb|center|'''Fig 1'''.The design result of the RBS in Part:BBa K2276007.]] | + | [[File:2017 SCU-China P1.png|1200px|thumb|center|'''Fig 1'''.The design result of the RBS in Part:BBa K2276007.]] |
− | [[File: | + | [[File:2017 SCU-China B0034.png|600px|thumb|center|'''Fig 2'''.The prediction of the translation rate of Part:BBa I13521]] |
===Source=== | ===Source=== |
Latest revision as of 07:33, 31 October 2017
a composite of TetR repressible promoter、special designed RBS and the coding sequence of mRFP
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 634
Illegal AgeI site found at 746 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We want to engineer a strain which can produce melatonin at daily rhythm.We employ repressilator system thus allowing the E.coli coding enzyme periodically. However, the cycle time is not 24h.We built a model to analyse the issue.According to the medeling result, a stronger RBS was needed.We turned to the bioinformatic methods to design a RBS predicted to show hign translation rate.This is the website on which we performed the design: https://salislab.net/software/doForwardRBS. We designed three, and BBa_K2276007 is one of them.
Source
Part:BBa_I13521
References
1.Borujeni A E, Salis H M. Translation Initiation is Controlled by RNA Folding Kinetics via a Ribosome Drafting Mechanism[J]. Journal of the American Chemical Society, 2016, 138(22):7016.
2.https://parts.igem.org/wiki/index.php?title=Part:BBa_I13521