Difference between revisions of "Part:BBa K2365033"
 
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PDC1 Yeast promoter;
 
PDC1 Yeast promoter;
 
Constitutive expression;
 
Constitutive expression;
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come from Saccharomyces cerevisiae BY4742 genomic sequence
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come from Saccharomyces cerevisiae BY4742 genomic sequence;
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pyruvate decarboxylase promoter, can be applied to the formation of CO2 pyruvate and acetaldehyde, relating to a stage of alcohol fermentation, exists in yeast and plants. Widely in a-ketoacid and aldehyde.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
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<partinfo>BBa_K2365033 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2365033 SequenceAndFeatures</partinfo>
  
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<!-- Uncomment this to enable Functional Parameter display
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K2365033 parameters</partinfo>
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<partinfo>BBa_K2365036 parameters</partinfo>
 
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[[File:TEF1 TEF1-cyc1.jpeg|350px|center]]
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PCR result
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[[File:Pdc1 pdc1-cyc1.jpeg|300px|center]]
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[[File:启动子全部.jpeg|400px|center]]
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Figure.The fluorescence of the four test devices and control transformed into Saccharomyces cerevisiae and inoculated in YPD broth was measured after 8h. 
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Nevertheless, the attractiveness of yeast as a platform organism (especially for metabolic engineering applications) needs continued efforts to expand the set of tools available.

Latest revision as of 00:49, 2 November 2017


PDC1 promotor

PDC1 Yeast promoter; Constitutive expression; come from Saccharomyces cerevisiae BY4742 genomic sequence;

pyruvate decarboxylase promoter, can be applied to the formation of CO2 pyruvate and acetaldehyde, relating to a stage of alcohol fermentation, exists in yeast and plants. Widely in a-ketoacid and aldehyde.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 289


PCR result

Pdc1 pdc1-cyc1.jpeg
启动子全部.jpeg

Figure.The fluorescence of the four test devices and control transformed into Saccharomyces cerevisiae and inoculated in YPD broth was measured after 8h. Nevertheless, the attractiveness of yeast as a platform organism (especially for metabolic engineering applications) needs continued efforts to expand the set of tools available.