Difference between revisions of "Part:BBa K2440029"

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MiRNA locker assembled by using this modularized DNA part was able to bind miR-458 in an Ago2 dependent manner, that is, knockdown of miR-458 was achieved by transfecting cells with miRNA locker.
 
MiRNA locker assembled by using this modularized DNA part was able to bind miR-458 in an Ago2 dependent manner, that is, knockdown of miR-458 was achieved by transfecting cells with miRNA locker.
  
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Dre-miR-458-5p is a novel RNA founded in zebrafish. And its function is still unknown.1
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Dre-miR-458-5p is a novel RNA founded in zebrafish<sup>1</sup>. And its function is still unknown.
  
 
===Sequence and Features===
 
===Sequence and Features===
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This part is sequenced as correct after construction.
 
This part is sequenced as correct after construction.
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==Reference==
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1.The developmental miRNA profiles of zebrafish as determined by small RNA cloning. Chen PY, Manninga H, Slanchev K, Chien M, Russo JJ, Ju J, Sheridan R, John B, Marks DS, Gaidatzis D, Sander C, Zavolan M, Tuschl T Genes Dev. 19:1288-1293(2005).

Latest revision as of 01:16, 1 November 2017


miR-458 target sequence

It is the target sequence of miR-458, a modularized DNA part from a set of chemically synthetic oligo DNA library.

Usage and Biology

MiRNA locker assembled by using this modularized DNA part was able to bind miR-458 in an Ago2 dependent manner, that is, knockdown of miR-458 was achieved by transfecting cells with miRNA locker.

Dre-miR-458-5p is a novel RNA founded in zebrafish1. And its function is still unknown.

Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Experimental Validation

This part is sequenced as correct after construction.


Reference

1.The developmental miRNA profiles of zebrafish as determined by small RNA cloning. Chen PY, Manninga H, Slanchev K, Chien M, Russo JJ, Ju J, Sheridan R, John B, Marks DS, Gaidatzis D, Sander C, Zavolan M, Tuschl T Genes Dev. 19:1288-1293(2005).