Difference between revisions of "Part:BBa K2440013"

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MiRNA locker assembled by using this modularized DNA part was able to bind miR-16 in an Ago2 dependent manner, that is, knockdown of miR-16 was achieved by transfecting cells with miRNA locker.
 
MiRNA locker assembled by using this modularized DNA part was able to bind miR-16 in an Ago2 dependent manner, that is, knockdown of miR-16 was achieved by transfecting cells with miRNA locker.
  
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MiR-16 was firstly detected in mouse kidney, adult zebrafish, and very weakly in frog ovary1. Located at chromosome 13q12, it is encoded by the mir-15a/mir16-1 and the mir-15b/mir16-2polycistrons.And its relative cloning frequency varied greatly and was higher in tumor samples and cell lines compared to normal tissues3.
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MiR-16 was firstly detected in mouse kidney, adult zebrafish, and very weakly in frog ovary<sup>1</sup>. Located at chromosome 13q1<sup>2</sup>, it is encoded by the mir-15a/mir16-1 and the mir-15b/mir16-2polycistrons.And its relative cloning frequency varied greatly and was higher in tumor samples and cell lines compared to normal tissues<sup>3</sup>.
  
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Reductions in expression of miR-15a and miR-16, let-7a, or miR-143 and miR-145 have been reported in chronic lymphocytic leukemia , lung cancer , and colorectal neoplasia , respectively4.
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Reductions in expression of miR-15a and miR-16, let-7a, or miR-143 and miR-145 have been reported in chronic lymphocytic leukemia , lung cancer , and colorectal neoplasia , respectively<sup>4</sup>.
  
 
===Sequence and Features===
 
===Sequence and Features===
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This part is sequenced as correct after construction.
 
This part is sequenced as correct after construction.
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 +
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==Reference==
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 +
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1.Identification of novel genes coding for small expressed RNAs. Lagos-Quintana M, Rauhut R, Lendeckel W, Tuschl T Science. 294:853-858(2001).
 +
 +
2.Frequent deletions and down-regulation of micro- RNA genes miR15 and miR16 at 13q14 in chronic lymphocytic leukemia. Calin GA, Dumitru CD, Shimizu M, Bichi R, Zupo S, Noch E, Aldler H, Rattan S, Keating M, Rai K, Rassenti L, Kipps T, Negrini M, Bullrich F, Croce CM Proc Natl Acad Sci U S A. 99:15524-15529(2002).
 +
 +
3.A mammalian microRNA expression atlas based on small RNA library sequencing. Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).
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 +
4.Patterns of known and novel small RNAs in human cervical cancer. Lui WO, Pourmand N, Patterson BK, Fire A Cancer Res. 67:6031-6043(2007).

Latest revision as of 01:34, 1 November 2017


miR-16 target sequence

It is the target sequence of miR-16, a modularized DNA part from a set of chemically synthetic oligo DNA library.

Usage and Biology

MiRNA locker assembled by using this modularized DNA part was able to bind miR-16 in an Ago2 dependent manner, that is, knockdown of miR-16 was achieved by transfecting cells with miRNA locker.

MiR-16 was firstly detected in mouse kidney, adult zebrafish, and very weakly in frog ovary1. Located at chromosome 13q12, it is encoded by the mir-15a/mir16-1 and the mir-15b/mir16-2polycistrons.And its relative cloning frequency varied greatly and was higher in tumor samples and cell lines compared to normal tissues3.

Reductions in expression of miR-15a and miR-16, let-7a, or miR-143 and miR-145 have been reported in chronic lymphocytic leukemia , lung cancer , and colorectal neoplasia , respectively4.

Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Experimental Validation

This part is sequenced as correct after construction.


Reference

1.Identification of novel genes coding for small expressed RNAs. Lagos-Quintana M, Rauhut R, Lendeckel W, Tuschl T Science. 294:853-858(2001).

2.Frequent deletions and down-regulation of micro- RNA genes miR15 and miR16 at 13q14 in chronic lymphocytic leukemia. Calin GA, Dumitru CD, Shimizu M, Bichi R, Zupo S, Noch E, Aldler H, Rattan S, Keating M, Rai K, Rassenti L, Kipps T, Negrini M, Bullrich F, Croce CM Proc Natl Acad Sci U S A. 99:15524-15529(2002).

3.A mammalian microRNA expression atlas based on small RNA library sequencing. Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).

4.Patterns of known and novel small RNAs in human cervical cancer. Lui WO, Pourmand N, Patterson BK, Fire A Cancer Res. 67:6031-6043(2007).