Difference between revisions of "Part:BBa K2271062:Design"
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===Design Notes===
 
===Design Notes===
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The part was designed as a membrane anchor for the v-SNARE Snc1 to secrete peroxisomes.
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The part was designed as a membrane marker for peroxisomes in <i> S. cerevisiea </i>.
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===Source===
 
===Source===
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The Promoter, mTurquoise and Terminator were obtained from A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly from Lee <i>et al.</i> (2015). [1]
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<br>
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Pex13 was synthesized by IDT.
  
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Synthesized by IDT.
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===References===
  
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===References===
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[1] <b>A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly </b> (2015)  <br>
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Michael E. Lee, William C. DeLoache, Bernardo Cervantes, and John E. Dueber <br>
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ACS Synth. Biol., 2015, 4 (9), pp 975–986 DOI: 10.1021/sb500366v

Latest revision as of 09:54, 31 October 2017

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal suffix found in sequence at 1987
    Illegal BglII site found at 880
    Illegal BglII site found at 917
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

The part was designed as a membrane marker for peroxisomes in S. cerevisiea .

Source

The Promoter, mTurquoise and Terminator were obtained from A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly from Lee et al. (2015). [1]
Pex13 was synthesized by IDT.

References

[1] A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly (2015)
Michael E. Lee, William C. DeLoache, Bernardo Cervantes, and John E. Dueber
ACS Synth. Biol., 2015, 4 (9), pp 975–986 DOI: 10.1021/sb500366v