Difference between revisions of "Part:BBa K2286005"

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<partinfo>BBa_K2286005 short</partinfo>
 
<partinfo>BBa_K2286005 short</partinfo>
  
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This part contains a root-specific promoter from Arabidopsis thaliana root and Hypocotyls. Compared to the full-length promoter,  
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This part contains a root-specific promoter from <em>Arabidopsis thaliana</em> root and Hypocotyls. Compared to the full-length promoter,  
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PYK10-1457 lacks the 785 bp base at the 5 'end , but its strength is increased by several times
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PYK10-1457 lacks the 785 bp base at the 5 'end , but its strength is increased by several times.
  
 
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To confirm whether the PPYK10-1457 promoter was strictly root-specific, we tested the roots and leaves of positive plants with RT-PCR.  
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To confirm whether the PPYK10-1457 promoter was strictly root-specific, we tested the roots and leaves of positive plants with RT-PCR.  
 
The results showed that the target gene CKX3 mainly express in the roots, only small amount of expression in leaves, indicating that  
 
The results showed that the target gene CKX3 mainly express in the roots, only small amount of expression in leaves, indicating that  
 
PYK10-1457 is an excellent root-specific promoter.  
 
PYK10-1457 is an excellent root-specific promoter.  
  
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[[File:T--UESTC-China--PYK10-1457-RT-PCR.png|500px|thumb|center|'''Fig 1'''.RT-PCR of CKX3 activated by PYK10-1457.]]
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[[File:T--UESTC-China--PYK10-1457-RT-PCR-2.png|800px|thumb|center|'''Fig.1''' RT-PCR of CKX3 activated by PYK10-1457.]]
  
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'''References'''
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1.Nitz, I., et al., Pyk10, a seedling and root specific gene and promoter from <em>Arabidopsis thaliana</em>. Plant Sci, 2001. 161(2): p. 337-346.
  
 
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Latest revision as of 17:43, 1 November 2017


PYK10-1457：a seedling and root specific promoter

This part contains a root-specific promoter from Arabidopsis thaliana root and Hypocotyls. Compared to the full-length promoter, PYK10-1457 lacks the 785 bp base at the 5 'end , but its strength is increased by several times.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

To confirm whether the PPYK10-1457 promoter was strictly root-specific, we tested the roots and leaves of positive plants with RT-PCR. The results showed that the target gene CKX3 mainly express in the roots, only small amount of expression in leaves, indicating that PYK10-1457 is an excellent root-specific promoter.

Fig.1 RT-PCR of CKX3 activated by PYK10-1457.


References

1.Nitz, I., et al., Pyk10, a seedling and root specific gene and promoter from Arabidopsis thaliana. Plant Sci, 2001. 161(2): p. 337-346.