Difference between revisions of "Part:BBa K2492000"
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<partinfo>BBa_K2492000 short</partinfo> | <partinfo>BBa_K2492000 short</partinfo> | ||
| − | This part arises from BBa_K309016[https://parts.igem.org/wiki/index.php?title=Part: | + | This part arises from BBa_K309016[https://parts.igem.org/wiki/index.php?title=Part:BBa_K309016], which is improved and characterized by our group. |
Modified GFP with three introns which can help well expression of GFP in C.elegans. In our group, we use it to indicate the location of neuron AWB in C.elegans. We integrated str1::CoCHR::GEM-GECO::GFP transgene in C.elegans by microinjection. Modified GFP will be highly expressed in AWB driven by promoter str1(no biobrick part). | Modified GFP with three introns which can help well expression of GFP in C.elegans. In our group, we use it to indicate the location of neuron AWB in C.elegans. We integrated str1::CoCHR::GEM-GECO::GFP transgene in C.elegans by microinjection. Modified GFP will be highly expressed in AWB driven by promoter str1(no biobrick part). | ||
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| − | [[File: T--SUSTech_Shenzhen-- | + | [[File: T--SUSTech_Shenzhen--C.elegans_AWB_GFP.png|800px|thumb|left|GFP expressed in AWB|GFP expressed in AWB]] |
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===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K2492000 parameters</partinfo> | <partinfo>BBa_K2492000 parameters</partinfo> | ||
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Latest revision as of 16:21, 1 November 2017
GFP (optimised, for use in the C. elegans chassis)
This part arises from BBa_K309016[1], which is improved and characterized by our group. Modified GFP with three introns which can help well expression of GFP in C.elegans. In our group, we use it to indicate the location of neuron AWB in C.elegans. We integrated str1::CoCHR::GEM-GECO::GFP transgene in C.elegans by microinjection. Modified GFP will be highly expressed in AWB driven by promoter str1(no biobrick part).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 264
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 797
Test for GFP: localize the neuron-AWB
We successfully ligated the str-1::Chrimson::GEM-GECO::GFP and injected the plasmids into C.elegans through microinjection. This picture was one of our stable inheritance strains. We used 488nm to excite AWB and observe at 500-540nm wavelength through confocal. We successfully observed a single green slender neuron on the anterior region of C.elegans. Comparing to the normal localisation of AWB, we indicated that the fluoresecence of GFP appeared in AWB. Thus we successfully used GFP to localise the position of target neuron.
