Difference between revisions of "Part:BBa K2336026:Design"
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===Design Notes=== | ===Design Notes=== | ||
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| + | We amplificated the oprF and FLAG-LBT separately as the amount of gene synthesized by IDT is too little.Then,we linked these two genes and got the plasmid with these two genes. | ||
===Source=== | ===Source=== | ||
| − | Synthesized by IDT. | + | Synthesized by IDT.And we fused 2 sequences(oprF and 3*LBT). |
===References=== | ===References=== | ||
Latest revision as of 17:21, 31 October 2017
oprF-GS-FLAG-LBT1-GS-LBT1-GS-LBT1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 72
Illegal NheI site found at 749 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 639
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 3
Illegal AgeI site found at 933 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We amplificated the oprF and FLAG-LBT separately as the amount of gene synthesized by IDT is too little.Then,we linked these two genes and got the plasmid with these two genes.
Source
Synthesized by IDT.And we fused 2 sequences(oprF and 3*LBT).