Difference between revisions of "Part:BBa K2323000"
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===Usage and Biology=== | ===Usage and Biology=== | ||
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===Plasmid composition=== | ===Plasmid composition=== | ||
[[File:BBa_K2323000.png| frame | 400px | center | The Biobrick contains the Lwa coding sequence and the Tphi terminator.([https://benchling.com/s/seq-IA6dwUFOSs7Tu0prM4VE read-only benchling map]) ]] | [[File:BBa_K2323000.png| frame | 400px | center | The Biobrick contains the Lwa coding sequence and the Tphi terminator.([https://benchling.com/s/seq-IA6dwUFOSs7Tu0prM4VE read-only benchling map]) ]] | ||
| + | The sequence is taken from J. S. Gootenberg et al., Science 10.1126/science.aam9321 (2017) | ||
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Latest revision as of 13:12, 30 October 2017
Lwa Cas13a + Tphi terminator
Cas13a is a CRISPR/Cas effector that specifically binds and digests RNA based on its crRNA sequence. After digestion of this target sequence, Cas13a undergoes conformation change and presents collateral activity, i.e. it indiscriminately digests RNA molecules.
The coding sequence is followed by Tphi terminator.
For characterization, see the full construct: BBa_K2323004. Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1286
Illegal BglII site found at 2966 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2365
Plasmid composition
The Biobrick contains the Lwa coding sequence and the Tphi terminator.(read-only benchling map)
The sequence is taken from J. S. Gootenberg et al., Science 10.1126/science.aam9321 (2017)