Difference between revisions of "Part:BBa K2311000"
 
(5 intermediate revisions by the same user not shown)
Line 3: Line 3:
 
<partinfo>BBa_K2311000 short</partinfo>
 
<partinfo>BBa_K2311000 short</partinfo>
  
−
This part has a positive feedback loop, so the expression of GFP can enhance quickly. In General, a positive feedback loop can be triggered by a low input such as PCR. This positive feedback loop will be opened by background expression of pLuxR in E.coli. But different input lead to different opening time.
+
This part has a positive feedback loop, so the expression of GFP can enhance quickly. But because of the LVA tag and LuxR's bug [http://2017.igem.org/Team:SiCAU-China/Contribution (more details about LuxR's bug)], this system could not work unless adding AHL (the concentration of 10^-9M may adequate in BL21).
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 07:54, 31 October 2017


Positive feedback loop (LuxI with LVA)

This part has a positive feedback loop, so the expression of GFP can enhance quickly. But because of the LVA tag and LuxR's bug [http://2017.igem.org/Team:SiCAU-China/Contribution (more details about LuxR's bug)], this system could not work unless adding AHL (the concentration of 10^-9M may adequate in BL21).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2315