Difference between revisions of "Part:BBa K2440007:Design"

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===Source===
 
===Source===
  
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We obtain it from PCR.
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We haven't obtained it sub-cloned onto the pSB1C3 plasmin backbone.
  
 
===References===
 
===References===
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[1] Mazumder B, Seshadri V, Fox PL (2003). "Translational control by the 3'-UTR: the ends specify the means". Trends Biochem. Sci. 28 (2): 91–8. PMID 12575997. DOI:10.1016/S0968-0004(03)00002-1
 
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[2] Ishikawa K, Nagase T, Suyama M, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O (Jun 1998). "Prediction of the coding sequences of unidentified human genes. X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro". DNA Research. 5 (3): 169–76. PMID 9734811. DOI:10.1093/dnares/5.3.169
 
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[3] Ramachandran S, Chahwan R, Nepal RM, Frieder D, Panier S, Roa S, Zaheen A, Durocher D, Scharff MD, Martin A (2010). "The RNF8/RNF168 ubiquitin ligase cascade facilitates class switch recombination". Proc Natl Acad. Sci. U.S.A. 107 (2): 809–14. PMC 2818930. PMID 20080757. DOI:10.1073/pnas.0913790107.
 
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[4] Xia Y, Yang W, Fa M, Li X, Wang Y, Jiang Y, Zheng Y, Lee JH, Li J, Lu Z (Jun 2017).” RNF8 mediates histone H3 ubiquitylation and promotes glycolysis and tumorigenesis.” J Exp Med. 214(6):1843-1855. PMID: 28507061 PMCID: PMC5461008 DOI: 10.1084/jem.20170015
 
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[5] Lu CS, Truong LN, Aslanian A, Shi LZ, Li Y, Hwang PY, Koh KH, Hunter T, Yates JR, Berns MW, Wu X (2012). "The RING finger protein RNF8 ubiquitinates Nbs1 to promote DNA double-strand break repair by homologous recombination". J Biol Chem. 287 (52): 43984–94. PMID: 23115235 PMCID: PMC3527981 DOI: 10.1074/jbc.M112.421545
 
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[6] Feng L, Chen J (2012). "The E3 ligase RNF8 regulates KU80 removal and NHEJ repair".Nat Struct Mol Biol. 19(2):201-6. PMID: 22266820 PMCID: PMC3888515 DOI: 10.1038/nsmb.2211
 
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[7] Marteijn JA, Bekker-Jensen S, Mailand N, Lans H, Schwertman P, Gourdin AM, Dantuma NP, Lukas J, Vermeulen W (2009). "Nucleotide excision repair-induced H2A ubiquitination is dependent on MDC1 and RNF8 and reveals a universal DNA damage response". J. Cell Biol. 186 (6): 835–47. PMID: 19797077 PMCID: PMC2753161 DOI: 10.1083/jcb.200902150
 
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[8] Wang M, Chen X, Chen H, Zhang X, Li J, Gong H, Shiyan C, Yang F (Jul 2015). “RNF8 plays an important role in the radioresistance of human nasopharyngeal cancer cells in vitro.” Oncol Rep. 34(1):341-9. PMID: 25955491 DOI: 10.3892/or.2015.3958.
 

Latest revision as of 23:02, 1 November 2017


RNF8 3-UTR


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1435
    Illegal XbaI site found at 3
    Illegal PstI site found at 637
    Illegal PstI site found at 2007
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1435
    Illegal PstI site found at 637
    Illegal PstI site found at 2007
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1435
    Illegal BglII site found at 927
    Illegal BglII site found at 1755
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1435
    Illegal XbaI site found at 3
    Illegal PstI site found at 637
    Illegal PstI site found at 2007
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1435
    Illegal XbaI site found at 3
    Illegal PstI site found at 637
    Illegal PstI site found at 2007
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1116


Design Notes

None.


Source

We haven't obtained it sub-cloned onto the pSB1C3 plasmin backbone.

References