Difference between revisions of "Part:BBa K2323014:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | no special design remarks. | + | no special design remarks. |
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
| − | This | + | This degradation tag is taken from Cameron & Collins, Tunable protein degradation in bacteria, Nature biotechnology, 32(12), December 2014. It can be degraded at a slow rate (0.0046/min) by the ClpP machinery of E.coli, but also at a faster rate by an exogenous protease (mf-lon). |
===References=== | ===References=== | ||
| + | Cameron & Collins, Tunable protein degradation in bacteria, Nature biotechnology, 32(12), December 2014 | ||
Latest revision as of 12:03, 30 October 2017
pdt2E degradation tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
no special design remarks.
Source
This degradation tag is taken from Cameron & Collins, Tunable protein degradation in bacteria, Nature biotechnology, 32(12), December 2014. It can be degraded at a slow rate (0.0046/min) by the ClpP machinery of E.coli, but also at a faster rate by an exogenous protease (mf-lon).
References
Cameron & Collins, Tunable protein degradation in bacteria, Nature biotechnology, 32(12), December 2014