Difference between revisions of "Part:BBa K2213001:Experience"

(→‎Applications of BBa_K2213001)
 
(2 intermediate revisions by one other user not shown)
Line 4: Line 4:
 
how you used this part and how it worked out.
 
how you used this part and how it worked out.
  
−
===Applications of BBa_K2213001===
+
=== Experience from UZurich 2019 ===
−
To find the optimal conditions of EutM microcompartment formation we used a tool called 'Design of Experiments' to vary a multitude of factors including:
+
 
−
- concentration of Tetracyclin inducer (induces EutMN synthesis)
+
When we characterized this part by redoing the OD measurement we found that the OD does not decrease drastically after 20 hours. Instead we observed a decline in growth during the log-phase that leveled out at an OD of around 1.5 which was still stable after 20 hours.<br>
−
- concentration of IPTG inducer (induces EutS synthesis)
+
 
−
- Harvest time (time after induction)
+
We therefore hypothesize that the production of the proteins EutM and EutN are detrimental to growth but not generally toxic to the cells, as Manchester proposed in 2017.
−
- Temperature
+
 
−
- Growth Medium (LB and TB)
+
===BBa_K2213001===
−
/
+
−
from this 22 flask investigation we were able to make surface plots to visulise our findings.
+
−
/
+
−
***2 TEMPERATURE DoE SURFACE PLOTS***
+
−
/
+
−
Figure1:
+
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 18:46, 20 October 2019


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Experience from UZurich 2019

When we characterized this part by redoing the OD measurement we found that the OD does not decrease drastically after 20 hours. Instead we observed a decline in growth during the log-phase that leveled out at an OD of around 1.5 which was still stable after 20 hours.

We therefore hypothesize that the production of the proteins EutM and EutN are detrimental to growth but not generally toxic to the cells, as Manchester proposed in 2017.

BBa_K2213001

User Reviews

UNIQa45ab8d9ba5deff0-partinfo-00000000-QINU UNIQa45ab8d9ba5deff0-partinfo-00000001-QINU