Difference between revisions of "Part:BBa K2333009:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | codon | + | The sequence for part has been codon-optimized for E. coli. |
| + | ===Source=== | ||
| + | mScarlet-I was originally designed by Bindels et al. 2016 "MScarlet: a bright monomeric red fluorescent protein for cellular imaging". This specific sequence was synthesized by IDT. | ||
| − | + | We use the website www.jcat.de to optimize the codon sequence for E. coli. | |
| − | + | ||
| − | + | ||
===References=== | ===References=== | ||
| + | [1] Bindels, D. S., Haarbosch, L., Weeren, L. V., Postma, M., Wiese, K. E., Mastop, M., . . . Gadella, T. W. (2016). MScarlet: a bright monomeric red fluorescent protein for cellular imaging. Nature Methods, 14(1), 53-56. doi:10.1038/nmeth.4074 | ||
Latest revision as of 20:17, 29 October 2017
mScarlet-I
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 512
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence for part has been codon-optimized for E. coli.
Source
mScarlet-I was originally designed by Bindels et al. 2016 "MScarlet: a bright monomeric red fluorescent protein for cellular imaging". This specific sequence was synthesized by IDT.
We use the website www.jcat.de to optimize the codon sequence for E. coli.
References
[1] Bindels, D. S., Haarbosch, L., Weeren, L. V., Postma, M., Wiese, K. E., Mastop, M., . . . Gadella, T. W. (2016). MScarlet: a bright monomeric red fluorescent protein for cellular imaging. Nature Methods, 14(1), 53-56. doi:10.1038/nmeth.4074