Difference between revisions of "Part:BBa I723024"
 
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<partinfo>BBa_I723143 short</partinfo>
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<partinfo>BBa_I723024 short</partinfo>
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This is the coding region for the first of two ''Pseudomonas aeruginosa'' PA01 enzymes required to convert phenazine-1-carboxylic acid (PCA) into pyocyanin.
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===Information contributed by City of London UK (2021)===
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Part information is collated here to help future users of the BioBrick registry.
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Metadata:
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*'''Group:''' City of London UK 2021
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*'''Author:''' Janusan Jeyananthan
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*'''Summary:''' Added information collated from existing scientific studies
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----
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PhzS required for activity in vitro.
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Enzyme catalytic activity: phenazine-1-carboxylate + S-adenosyl-L-methionine = 5-methyl-phenazine-1-carboxylate + S-adenosyl-L-homocysteine
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<ref>"phzM" n.d Uniprot. Accessed October 3, 2021. https://www.uniprot.org/uniprot/Q9HWH2</ref>
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[[File:phzM Reaction Diagram.JPG|x200px|center]]
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===References===
  
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This is the coding region for the first of two ''Pseudomonas aeruginosa'' PA01 enzymes required to convert phenazine-1-carboxylic acid (PCA) into pyocyanin
 
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_I723024 SequenceAndFeatures</partinfo>
  
  
 
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===Functional Parameters===
 
===Functional Parameters===
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Latest revision as of 12:55, 3 October 2021

PhzM

This is the coding region for the first of two Pseudomonas aeruginosa PA01 enzymes required to convert phenazine-1-carboxylic acid (PCA) into pyocyanin.

Information contributed by City of London UK (2021)

Part information is collated here to help future users of the BioBrick registry.

Metadata:

  • Group: City of London UK 2021
  • Author: Janusan Jeyananthan
  • Summary: Added information collated from existing scientific studies

PhzS required for activity in vitro.

Enzyme catalytic activity: phenazine-1-carboxylate + S-adenosyl-L-methionine = 5-methyl-phenazine-1-carboxylate + S-adenosyl-L-homocysteine [1]

PhzM Reaction Diagram.JPG

References

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 206
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 424
    Illegal NgoMIV site found at 428
    Illegal NgoMIV site found at 787
  • 1000
    COMPATIBLE WITH RFC[1000]


  1. ↑ "phzM" n.d Uniprot. Accessed October 3, 2021. https://www.uniprot.org/uniprot/Q9HWH2